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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2008-9-29
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pubmed:abstractText |
This study examined the efficacy of poly(NiPAAm-co-AAc) as an injectable drug delivery vehicle and a cell therapeutic agent in the form of a supporting matrix for the chondrogenic differentiation of rabbit chondrocytes. The hydrogel constructs, which consisted of embedded cells co-encapsulating dexamethasone (Dex) and TGF beta-1 or unloaded Dex, were used as controls to determine the effects of Dex and TGF beta-1 on chondrogenic differentiation. The level of Dex and TGF beta-1 released was monitored using a bioimaging method. The amount of Dex released from hydrogel was faster than that of TGF beta-1. TGF beta-1 was present in hydrogel for more than 4 weeks after the injection. The level of the cartilage associated ECM proteins was examined by immunohistochemical staining for collagen type II as well as by Safranin-O and Alcian blue (GAG) staining. These results highlight the potential of a thermo-reversible hydrogel mixed with the chondrocytes and differentiation delivery material for applications in neocartilage formation.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acrylic Resins,
http://linkedlifedata.com/resource/pubmed/chemical/Biocompatible Materials,
http://linkedlifedata.com/resource/pubmed/chemical/CY5.5 cyanine dye,
http://linkedlifedata.com/resource/pubmed/chemical/Carbocyanines,
http://linkedlifedata.com/resource/pubmed/chemical/Collagen Type II,
http://linkedlifedata.com/resource/pubmed/chemical/Dexamethasone,
http://linkedlifedata.com/resource/pubmed/chemical/Drug Carriers,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescein-5-isothiocyanate,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes,
http://linkedlifedata.com/resource/pubmed/chemical/Glucocorticoids,
http://linkedlifedata.com/resource/pubmed/chemical/Hydrogels,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta1,
http://linkedlifedata.com/resource/pubmed/chemical/poly(N-isopropylacrylamide-co-acryla...
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
1552-4965
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
87
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
283-9
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pubmed:meshHeading |
pubmed-meshheading:18181092-Acrylic Resins,
pubmed-meshheading:18181092-Animals,
pubmed-meshheading:18181092-Biocompatible Materials,
pubmed-meshheading:18181092-Carbocyanines,
pubmed-meshheading:18181092-Cell Differentiation,
pubmed-meshheading:18181092-Cells, Cultured,
pubmed-meshheading:18181092-Chondrocytes,
pubmed-meshheading:18181092-Chondrogenesis,
pubmed-meshheading:18181092-Collagen Type II,
pubmed-meshheading:18181092-Dexamethasone,
pubmed-meshheading:18181092-Drug Carriers,
pubmed-meshheading:18181092-Female,
pubmed-meshheading:18181092-Fluorescein-5-isothiocyanate,
pubmed-meshheading:18181092-Fluorescent Dyes,
pubmed-meshheading:18181092-Glucocorticoids,
pubmed-meshheading:18181092-Humans,
pubmed-meshheading:18181092-Hydrogels,
pubmed-meshheading:18181092-Male,
pubmed-meshheading:18181092-Materials Testing,
pubmed-meshheading:18181092-Mice,
pubmed-meshheading:18181092-Mice, Inbred BALB C,
pubmed-meshheading:18181092-Mice, Nude,
pubmed-meshheading:18181092-Rabbits,
pubmed-meshheading:18181092-Tissue Engineering,
pubmed-meshheading:18181092-Transforming Growth Factor beta1
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pubmed:year |
2008
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pubmed:articleTitle |
Bioimaging of dexamethasone and TGF beta-1 and its biological activities of chondrogenic differentiation in hydrogel constructs.
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pubmed:affiliation |
Division of Biotechnology, The Catholic University of Korea, Wonmi-gu, Bucheon 420-743, Korea.
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pubmed:publicationType |
Journal Article,
Evaluation Studies
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