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rdf:type |
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lifeskim:mentions |
umls-concept:C0007595,
umls-concept:C0013138,
umls-concept:C0035553,
umls-concept:C0076836,
umls-concept:C0220781,
umls-concept:C0851285,
umls-concept:C1199517,
umls-concept:C1419603,
umls-concept:C1514873,
umls-concept:C1546857,
umls-concept:C1556066,
umls-concept:C1619636,
umls-concept:C1704259,
umls-concept:C1705987,
umls-concept:C1883254
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pubmed:issue |
6
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pubmed:dateCreated |
2007-12-18
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pubmed:abstractText |
Synthesis of ribosomal RNA (rRNA) is a key step in ribosome biogenesis and is essential for cell growth. Few studies, however, have investigated rRNA synthesis regulation in vivo in multicellular organisms. Here, we present a genetic analysis of transcription initiation factor IA (TIF-IA), a conserved RNA polymerase I transcription factor. Drosophila melanogaster Tif-IA(-/-) mutants have reduced levels of rRNA synthesis and sustain a developmental arrest caused by a block in cellular growth. We find that the target of rapamycin (TOR) pathway regulates TIF-IA recruitment to rDNA. Furthermore, we show that the TOR pathway regulates rRNA synthesis in vivo and that TIF-IA overexpression can maintain rRNA transcription when TOR activity is reduced in developing larvae. We propose that TIF-IA acts in vivo as a downstream growth-regulatory target of the TOR pathway. Overexpression of TIF-IA also elevates levels of both 5S RNA and messenger RNAs encoding ribosomal proteins. Stimulation of rRNA synthesis by TIF-IA may therefore provide a feed-forward mechanism to coregulate the levels of other ribosome components.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/18086911-10198052,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18086911-10497130,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18086911-10559149,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18086911-10748524,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18086911-10758157,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18086911-11069885,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18086911-11069888,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18086911-11250903,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18086911-11265758,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18086911-11389445,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18086911-11717393,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18086911-12015311,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18086911-12766776,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18086911-12865296,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18086911-14595104,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18086911-14609951,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18086911-14612424,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18086911-14688273,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18086911-14712261,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18086911-15004009,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18086911-15196469,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18086911-15568992,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18086911-15691654,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18086911-15723055,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18086911-15989966,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18086911-16157872,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18086911-16469695,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18086911-16882981,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18086911-16888624,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18086911-16900101,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18086911-17308101,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18086911-17371599,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18086911-9671456
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Ribosomal,
http://linkedlifedata.com/resource/pubmed/chemical/Drosophila Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositol 3-Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Ribosomal,
http://linkedlifedata.com/resource/pubmed/chemical/RNA Precursors,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/target of rapamycin protein...
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
1540-8140
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
17
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pubmed:volume |
179
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1105-13
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:18086911-Animals,
pubmed-meshheading:18086911-Cell Enlargement,
pubmed-meshheading:18086911-DNA, Ribosomal,
pubmed-meshheading:18086911-Drosophila Proteins,
pubmed-meshheading:18086911-Drosophila melanogaster,
pubmed-meshheading:18086911-Larva,
pubmed-meshheading:18086911-Mutation,
pubmed-meshheading:18086911-Phosphatidylinositol 3-Kinases,
pubmed-meshheading:18086911-Protein Kinases,
pubmed-meshheading:18086911-RNA, Messenger,
pubmed-meshheading:18086911-RNA, Ribosomal,
pubmed-meshheading:18086911-RNA Precursors,
pubmed-meshheading:18086911-Ribosomes,
pubmed-meshheading:18086911-Signal Transduction,
pubmed-meshheading:18086911-Transcription, Genetic,
pubmed-meshheading:18086911-Transcription Factors
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pubmed:year |
2007
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pubmed:articleTitle |
Drosophila TIF-IA is required for ribosome synthesis and cell growth and is regulated by the TOR pathway.
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pubmed:affiliation |
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA. grewalss@ucalgary.ca
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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