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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
2007-11-29
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pubmed:abstractText |
For the preparation of the complex of IGF-II and IGFBP-6, a co-expression vector containing two copies of human IGF-II and IGFBP-6 expression cassette was constructed with alcohol oxidase (AOX1) promoter and secretion signal sequence of alpha-factor, and transformed to Pichia pastoris yeast. Through a purification procedure involving anion-exchange chromatography and gel filtration, a complex of IGF-II with IGFBP-6 was obtained. An additional C-terminal sequence of IGFBP-6 (CS-BP6) was found to be bound to this complex. Dynamic light scattering showed that this complex was very stable and homogenous in solution. Western blotting based on non-reducing Tricine-SDS-PAGE indicated that IGF-II expression coupled with IGFBP-6 might significantly avoid the mispairing of disulfide bonds compared with the IGF-II expressed alone.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
0929-8665
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
14
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
876-80
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pubmed:meshHeading |
pubmed-meshheading:18045229-Base Sequence,
pubmed-meshheading:18045229-Blotting, Western,
pubmed-meshheading:18045229-Chromatography, Ion Exchange,
pubmed-meshheading:18045229-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:18045229-Gene Expression,
pubmed-meshheading:18045229-Genetic Vectors,
pubmed-meshheading:18045229-Humans,
pubmed-meshheading:18045229-Insulin-Like Growth Factor Binding Protein 6,
pubmed-meshheading:18045229-Insulin-Like Growth Factor II,
pubmed-meshheading:18045229-Nucleic Acid Conformation,
pubmed-meshheading:18045229-Pichia,
pubmed-meshheading:18045229-Plasmids,
pubmed-meshheading:18045229-Protein Folding,
pubmed-meshheading:18045229-Recombinant Fusion Proteins
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pubmed:year |
2007
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pubmed:articleTitle |
Co-expression and purification of recombinant human insulin-like growth factor II and insulin-like growth factor binding protein-6 in Pichia pastoris yeast.
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pubmed:affiliation |
Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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