Linked Life Data

17981875

Source:http://linkedlifedata.com/resource/pubmed/id/17981875

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2007-12-12
pubmed:abstractText
We describe here a novel proteoliposome reconstitution system for functional analysis of plant membrane transporters that is based on a modified wheat germ cell-free translation system. We established optimized conditions for the reconstitution system with Arabidopsis thaliana phosphoenolpyruvate/phosphate translocator 1 (AtPPT1) as a model transporter. A high activity of AtPPT1 was achieved by synthesis of the protein in the presence of both a detergent such as Brij35 and liposomes. We also determined the substrate specificities of three putative rice PPT homologs with this system. The cell-free proteoliposome reconstitution system provides a valuable tool for functional analysis of transporter proteins.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0032-0781
pubmed:author
pubmed:issnType
Print
pubmed:volume
48
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1815-20
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
A cell-free translation and proteoliposome reconstitution system for functional analysis of plant solute transporters.
pubmed:affiliation
Cell-Free Science and Technology Research Center, and The Venture Business Laboratory, Ehime University, Matsuyama, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't