Source:http://linkedlifedata.com/resource/pubmed/id/17974952
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
21
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pubmed:dateCreated |
2007-11-2
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pubmed:abstractText |
MicroRNAs (miRNA) are endogenous noncoding small RNAs that regulate the activity of mRNAs. Many miRNA genes, including let-7a-3, are located in CpG islands, suggesting possible epigenetic regulation of their expression. Promoter CpG island methylation of tumor suppressor genes is involved in cancer development and progression. Using real-time methylation-specific PCR and real-time reverse transcription-PCR, we analyzed DNA methylation in the let-7a-3 gene and miRNA expression of let-7a in 214 patients with epithelial ovarian cancer to assess the effect of let-7a-3 methylation on the expressions of let-7a as well as a possible target of let-7 regulation, insulin-like growth factor-II (IGF-II). The association of let-7a-3 methylation with patient survival outcomes was also evaluated. let-7a-3 methylation was detected in epithelial ovarian cancer, and the expression of let-7a was slightly affected by the methylation, but the effect was not substantial. The methylation of let-7a-3, however, was inversely correlated with IGF-II expression and positively with insulin-like growth factor binding protein-3 (IGFBP-3) expression. Patients with methylated let-7a-3 seemed to have reduced risk for death compared with those without, and the association was independent of patient age at surgery, tumor grade, disease stage, and IGF-II or IGFBP-3 expression. No association was found for let-7a-3 methylation and disease progression. These results suggest that the let-7a-3 gene is methylated and the methylation may affect IGF-II expression and the survival of ovarian cancer patients. Further investigation of the role of miRNAs and their regulation in cancer is warranted.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/IGFBP3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin-Like Growth Factor Binding...,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin-Like Growth Factor Binding...,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin-Like Growth Factor II,
http://linkedlifedata.com/resource/pubmed/chemical/MicroRNAs,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/mirnlet7 microRNA, human
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
1538-7445
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:day |
1
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pubmed:volume |
67
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
10117-22
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:17974952-Adult,
pubmed-meshheading:17974952-Aged,
pubmed-meshheading:17974952-Aged, 80 and over,
pubmed-meshheading:17974952-DNA Methylation,
pubmed-meshheading:17974952-Female,
pubmed-meshheading:17974952-Humans,
pubmed-meshheading:17974952-Insulin-Like Growth Factor Binding Protein 3,
pubmed-meshheading:17974952-Insulin-Like Growth Factor Binding Proteins,
pubmed-meshheading:17974952-Insulin-Like Growth Factor II,
pubmed-meshheading:17974952-MicroRNAs,
pubmed-meshheading:17974952-Middle Aged,
pubmed-meshheading:17974952-Neoplasms, Glandular and Epithelial,
pubmed-meshheading:17974952-Ovarian Neoplasms,
pubmed-meshheading:17974952-Prognosis,
pubmed-meshheading:17974952-RNA, Messenger
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pubmed:year |
2007
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pubmed:articleTitle |
Hypermethylation of let-7a-3 in epithelial ovarian cancer is associated with low insulin-like growth factor-II expression and favorable prognosis.
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pubmed:affiliation |
Department of Epidemiology and Public Health, Yale Cancer Center, Yale University School of Medicine, New Haven, CT 06520-8034, USA.
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pubmed:publicationType |
Journal Article
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