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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
21
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pubmed:dateCreated |
2007-10-31
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pubmed:abstractText |
Proteomics has grown significantly with the aid of new technologies that consistently are becoming more streamlined. While processing of proteins from a whole cell lysate is typically done in a bottom-up fashion utilizing MS/MS of peptides from enzymatically digested proteins, top-down proteomics is becoming a viable alternative that until recently has been limited largely to offline analysis by tandem mass spectrometry. Here we describe a method for high-resolution tandem mass spectrometery of intact proteins on a chromatographic time scale. In a single liquid chromatography-tandem mass spectrometry (LC-MS/MS) run, we have identified 22 yeast proteins with molecular weights from 14 to 35 kDa. Using anion exchange chromatography to fractionate a whole cell lysate before online LC-MS/MS, we have detected 231 metabolically labeled (14N/15N) protein pairs from Saccharomyces cerevisiae. Thirty-nine additional proteins were identified and characterized from LC-MS/MS of selected anion exchange fractions. Automated localization of multiple acetylations on Histone H4 was also accomplished on an LC time scale from a complex protein mixture. To our knowledge, this is the first demonstration of top-down proteomics (i.e., many identifications) on linear ion trap Fourier transform (LTQ FT) systems using high-resolution MS/MS data obtained on a chromatographic time scale.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/17915963-10359756,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17915963-10757168,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17915963-10786879,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17915963-11287652,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17915963-11354501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17915963-11774908,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17915963-11788986,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17915963-12124999,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17915963-12298093,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17915963-12645901,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17915963-14632120,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17915963-14750868,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17915963-14976258,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17915963-15340378,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17915963-15384137,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17915963-15707830,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17915963-15983376,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17915963-16236702,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17915963-16253516,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17915963-16448040,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17915963-16457587,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17915963-16457589,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17915963-16574746,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17915963-16772433,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17915963-16808433,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17915963-16835692,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17915963-16955517,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17915963-17031791,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17915963-17269717,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17915963-17287358,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17915963-236308,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17915963-8703047
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0003-2700
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
79
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7984-91
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pubmed:dateRevised |
2010-8-17
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pubmed:meshHeading |
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pubmed:year |
2007
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pubmed:articleTitle |
Top-down proteomics on a chromatographic time scale using linear ion trap fourier transform hybrid mass spectrometers.
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pubmed:affiliation |
Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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