Linked Life Data

17911357

Source:http://linkedlifedata.com/resource/pubmed/id/17911357

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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2008-1-25
pubmed:abstractText
Parathyroid hormone-related peptide (PTHrP) is released under ischaemic conditions and it improves contractile function of stunned myocardium. The actions of PTHrP are mediated primarily by the type 1 parathyroid hormone receptor (PTH.1R), while PTHrP and PTH.1R expression levels are increased in ventricular hypertrophy associated with experimental hyperthyroidism. Since chronic administration of thyroxine (T4) improves postischaemic recovery in isolated heart models subjected to ischaemia-reperfusion stress, we tested the hypothesis that experimentally induced hyperthyroidism is associated with elevated expression of PTHrP and PTH.1R in rat myocardium. Hyperthyroid and control male Wistar rats were subjected to ischaemia-reperfusion stress using the Langendorff technique, and the PTHrP and PTH.1R expression was assessed by relative quantitative reverse transcriptase-polymerase chain reaction, Western blot analysis and immunohistochemistry. In the Langendorff model, the recovery of left ventricular developed pressure at the end of the stablization period and 45 min into the reperfusion period was used to assess the cardioprotective actions of T4 administration. Our data show that hyperthyroid animals had increased tolerance to the ischaemia-reperfusion stress and that this was associated with an increase of PTHrP and PTH.1R expression levels compared with those of control animals. In the control animals, the expression of PTHrP was increased 45 min into the reperfusion phase, while the PTH.1R expression pattern was significantly and gradually decreased throughout the ischaemia and reperfusion phases. In the hyperthyroid animals, the PTHrP and PTH.1R expression pattern was significantly higher throughout the ischaemia and reperfusion phases compared with that of control hearts. Our data suggest that increasing levels of PTHrP and PTH.1R expression can mediate, at least in part, the T4 administration-induced cardioprotection in rat ventricular myocardium.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0958-0670
pubmed:author
pubmed:issnType
Print
pubmed:volume
93
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
237-46
pubmed:meshHeading
pubmed-meshheading:17911357-Animals, pubmed-meshheading:17911357-Blotting, Western, pubmed-meshheading:17911357-DNA, Complementary, pubmed-meshheading:17911357-Heart Ventricles, pubmed-meshheading:17911357-Hyperthyroidism, pubmed-meshheading:17911357-Immunohistochemistry, pubmed-meshheading:17911357-Male, pubmed-meshheading:17911357-Myocardial Reperfusion Injury, pubmed-meshheading:17911357-Myocardium, pubmed-meshheading:17911357-Parathyroid Hormone-Related Protein, pubmed-meshheading:17911357-RNA, pubmed-meshheading:17911357-Rats, pubmed-meshheading:17911357-Rats, Wistar, pubmed-meshheading:17911357-Receptor, Parathyroid Hormone, Type 1, pubmed-meshheading:17911357-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:17911357-Thyroid Hormones, pubmed-meshheading:17911357-Thyroxine
pubmed:year
2008
pubmed:articleTitle
Experimental hyperthyroidism increases expression of parathyroid hormone-related peptide and type-1 parathyroid hormone receptor in rat ventricular myocardium of the Langendorff ischaemia-reperfusion model.
pubmed:affiliation
Department of Experimental Physiology, Medical School, University of Athens, 75 Micras Asias Goudi-Athens, 115 27 Greece.
pubmed:publicationType
Journal Article, In Vitro