|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0004381,
umls-concept:C0023884,
umls-concept:C0025914,
umls-concept:C0026809,
umls-concept:C0028630,
umls-concept:C0120465,
umls-concept:C0206588,
umls-concept:C1413828,
umls-concept:C1414250,
umls-concept:C1417827,
umls-concept:C1420354,
umls-concept:C1514559,
umls-concept:C1518440
|
|
pubmed:issue |
25
|
|
pubmed:dateCreated |
2007-10-12
|
|
pubmed:abstractText |
Various drugs such as phenobarbital (PB) trigger translocation of constitutive active/adrostane receptor (CAR) from the cytoplasm into the nucleus of mouse liver cells without directly binding to the receptor. We have now characterized the guanine nucleotide exchange factor epithelial cell-transforming gene 2 (ECT2) as a PB-inducible factor as well as a cellular signal that represses PB-triggered nuclear translocation of CAR. When CFP-tagged ECT2 was co-expressed with YFP-tagged CAR in the liver of Car(-/-) mice, ECT2 repressed CAR nuclear translocation. Coexpression of various deletion mutants delineated this repressive activity to the tandem Dbl homology/pleckstrin homology domains of ECT2 and to their cytosolic expression. CAR directly bound to the PH domain. Thus, ECT2 may comprise a part of the PB response signal regulating the intracellular trafficking of CAR.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/17904126-10037683,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17904126-10454578,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17904126-10854437,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17904126-11057673,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17904126-11134331,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17904126-11283262,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17904126-11752199,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17904126-12464260,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17904126-12637530,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17904126-14587037,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17904126-14645260,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17904126-15073184,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17904126-15340055,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17904126-15347657,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17904126-15492232,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17904126-16310787,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17904126-16492670,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17904126-17050775,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17904126-17284330,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17904126-17314319,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17904126-1846048,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17904126-3779841,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17904126-7623810,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17904126-8464478,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17904126-9742082
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Oct
|
|
pubmed:issn |
0014-5793
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
16
|
|
pubmed:volume |
581
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
4937-42
|
|
pubmed:dateRevised |
2011-9-26
|
|
pubmed:meshHeading |
pubmed-meshheading:17904126-Active Transport, Cell Nucleus,
pubmed-meshheading:17904126-Animals,
pubmed-meshheading:17904126-Binding Sites,
pubmed-meshheading:17904126-Cell Line, Tumor,
pubmed-meshheading:17904126-Cell Nucleus,
pubmed-meshheading:17904126-Guanine Nucleotide Exchange Factors,
pubmed-meshheading:17904126-Humans,
pubmed-meshheading:17904126-Liver,
pubmed-meshheading:17904126-Male,
pubmed-meshheading:17904126-Mice,
pubmed-meshheading:17904126-Mice, Knockout,
pubmed-meshheading:17904126-Phenobarbital,
pubmed-meshheading:17904126-Protein Structure, Tertiary,
pubmed-meshheading:17904126-Proto-Oncogene Proteins,
pubmed-meshheading:17904126-Receptors, Cytoplasmic and Nuclear,
pubmed-meshheading:17904126-Sequence Deletion,
pubmed-meshheading:17904126-Transcription, Genetic,
pubmed-meshheading:17904126-Transcription Factors
|
|
pubmed:year |
2007
|
|
pubmed:articleTitle |
Overexpression of the Rho-guanine nucleotide exchange factor ECT2 inhibits nuclear translocation of nuclear receptor CAR in the mouse liver.
|
|
pubmed:affiliation |
Pharmacogenetics Section, Laboratory of Reproductive and Developmental Toxicology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, United States.
|
|
pubmed:publicationType |
Journal Article
|
