Linked Life Data

1779092

Source:http://linkedlifedata.com/resource/pubmed/id/1779092

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1992-3-10
pubmed:abstractText
A method to detect metallothionein (MT) by Western blotting technique is described. Cadmium-containing rat MT-I and -II were subjected to sodium dodecylsulfate/polyacrylamide gel electrophoresis (SDS-PAGE) without prior reduction by 2-mercaptoethanol (2-ME), because prior reduction of MTs by 2-ME resulted in diffused bands. After SDS-PAGE, MTs in the gel were reduced by incubating in buffer containing 2-ME, and then electrophoretically transferred to a polyvinylidene fluoride membrane. Non-reduced MTs were not found to be immobilized on the membrane. Prior to electrophoresis the zinc, in zinc-containing MT samples, must be replaced with cadmium by addition of excess cadmium chloride. MTs immobilized on the membrane were detected by two methods, (1) binding of radioactive cadmium and (2) immunochemical staining using protein-A/colloidal gold conjugate, the detection limits of which were 0.4 and 0.06-0.16 micrograms, respectively.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0165-022X
pubmed:author
pubmed:issnType
Print
pubmed:volume
23
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
207-16
pubmed:dateRevised
2006-4-17
pubmed:meshHeading
pubmed:articleTitle
A western blotting procedure for detection of metallothionein.
pubmed:affiliation
National Institute for Environmental Studies, Ibaraki, Japan.
pubmed:publicationType
Journal Article