Linked Life Data

1772427

Source:http://linkedlifedata.com/resource/pubmed/id/1772427

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1992-2-24
pubmed:abstractText
Recently, an assay for quantification of glycosaminoglycans has been reported based on precipitation with Safranin O (Lammi, M. and Tammi, M. (1988) Anal. Biochem. 168, 352-357). In this procedure, the precipitate which forms when the glycosaminoglycan or proteoglycan is mixed with the Safranin O is collected with a dot-blot apparatus onto a membrane filter. The intensity of the color in the dots is measured densitometrically and is proportional to the amount of glycosaminoglycan or proteoglycan in the sample. This report describes a modification of the densitometric Safranin O assay which allows its use as a spectrophotometric assay. For this, the precipitates are solubilized in cetylpyridinium chloride and the absorbance determined for the resulting solutions. As with the densitometric method, guanidinium chloride diminishes the color intensity. However, the color is stable, even after solubilization, for at least one week. The precipitates collected from as much as 10 micrograms of material can be solubilized in as little as 100 microliters of cetylpyridinium chloride, so that increased sensitivity may be obtained if the solubilized precipitate is measured in a microcuvet. Thus, solubilization with cetylpyridinium chloride allows use of the Safranin O assay for glycosaminoglycans and proteoglycans even when a densitometer is unavailable.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0158-5231
pubmed:author
pubmed:issnType
Print
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
485-95
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
A spectrophotometric modification of a sensitive densitometric Safranin O assay for glycosaminoglycans.
pubmed:affiliation
Department of Biology, Case Western Reserve University, Cleveland, Ohio 44106.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.