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rdf:type |
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lifeskim:mentions |
umls-concept:C0001613,
umls-concept:C0002716,
umls-concept:C0007776,
umls-concept:C0022655,
umls-concept:C0025462,
umls-concept:C0027882,
umls-concept:C0053246,
umls-concept:C0085262,
umls-concept:C0235032,
umls-concept:C1280500,
umls-concept:C1527240,
umls-concept:C2000410
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pubmed:issue |
3
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pubmed:dateCreated |
2007-9-3
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pubmed:abstractText |
We have investigated the effect of KHG21834, a benzothiazole derivative, on the amyloid beta protein (Abeta)-induced cell death in rat pheochromocytoma (PC12) cells and rat cortical and mesencephalic neuron-glia cultures. KHG21834 attenuated the Abeta(25-35)-induced apoptotic death in PC12 cells determined by characteristic morphological alterations and positive in situ terminal end-labeling (TUNEL). In the cortical neuron-glia cultures, KHG21834 reduced the Abeta(25-35)-induced apoptosis determined by TUNEL staining. Immunocytochemical analysis and Western blot analysis of Abeta(25-35)-induced neurotoxicity in mesencephalic neuron-glia cultures with anti-tyrosine hydroxylase (TH) antibody showed that Abeta(25-35) decreased the expression of TH protein by 60% and KHG21834 significantly attenuated the Abeta(25-35)-induced reduction in the expression of TH. Moreover, KHG21834 attenuates Abeta(25-35)-induced toxicity concomitant with the reduction of activation of extracellular signal-regulated kinase (ERK)1/2 to a lesser extent. ERK1 was more sensitively affected than ERK2 in attenuation of Abeta(25-35)-induced phosphorylation by KHG21834. These results demonstrated that KHG21834 was capable of protecting neuronal cells from Abeta(25-35)-induced degeneration.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0300-483X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
8
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pubmed:volume |
239
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
156-66
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:17714846-Amyloid beta-Peptides,
pubmed-meshheading:17714846-Animals,
pubmed-meshheading:17714846-Antibodies,
pubmed-meshheading:17714846-Apoptosis,
pubmed-meshheading:17714846-Benzothiazoles,
pubmed-meshheading:17714846-Blotting, Western,
pubmed-meshheading:17714846-Cell Survival,
pubmed-meshheading:17714846-Cells, Cultured,
pubmed-meshheading:17714846-Cerebral Cortex,
pubmed-meshheading:17714846-DNA Fragmentation,
pubmed-meshheading:17714846-Immunohistochemistry,
pubmed-meshheading:17714846-In Situ Nick-End Labeling,
pubmed-meshheading:17714846-Mesencephalon,
pubmed-meshheading:17714846-Microscopy, Confocal,
pubmed-meshheading:17714846-Microscopy, Fluorescence,
pubmed-meshheading:17714846-Mitogen-Activated Protein Kinase 3,
pubmed-meshheading:17714846-Molecular Structure,
pubmed-meshheading:17714846-Neurons,
pubmed-meshheading:17714846-Neuroprotective Agents,
pubmed-meshheading:17714846-PC12 Cells,
pubmed-meshheading:17714846-Peptide Fragments,
pubmed-meshheading:17714846-Rats,
pubmed-meshheading:17714846-Rats, Sprague-Dawley,
pubmed-meshheading:17714846-Tyrosine 3-Monooxygenase
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pubmed:year |
2007
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pubmed:articleTitle |
Protective effect of benzothiazole derivative KHG21834 on amyloid beta-induced neurotoxicity in PC12 cells and cortical and mesencephalic neurons.
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pubmed:affiliation |
Department of Biochemistry and Molecular Biology, University of Ulsan College of Medicine, Seoul 138-736, Republic of Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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