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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2007-10-1
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pubmed:abstractText |
The cytotoxic effect of the tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is limited in some cancer cells, including AGS gastric adenocarcinoma cells. However, treatment with TRAIL in combination with subtoxic concentrations of genistein sensitizes TRAIL-resistant AGS cells to TRAIL-mediated apoptosis. Combined treatment with genistein and TRAIL-induced chromatin condensation and sub-G1 phase DNA content. These indicators of apoptosis are correlated with the activation of death receptors (DR5) and induction of caspase-3 activity, which results in the cleavage of poly(ADP-ribose)polymerase. Both the cytotoxic effect and apoptotic characteristics induced by combined treatment were significantly inhibited by z-DEVD-fmk, a caspase-3 inhibitor, which demonstrates the important role of caspase-3 in the observed cytotoxic effect. These results indicate that caspase-3 is a key regulator of apoptosis in response to combined genistein and TRAIL in human gastric adenocarcinoma AGS cells through the activation of DR5 and mitochondrial dysfunction.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0304-3835
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
8
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pubmed:volume |
257
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
56-64
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pubmed:meshHeading |
pubmed-meshheading:17689858-Adenocarcinoma,
pubmed-meshheading:17689858-Anticarcinogenic Agents,
pubmed-meshheading:17689858-Apoptosis,
pubmed-meshheading:17689858-Caspase 3,
pubmed-meshheading:17689858-Cell Line, Tumor,
pubmed-meshheading:17689858-Cell Nucleus,
pubmed-meshheading:17689858-Cells, Cultured,
pubmed-meshheading:17689858-Dose-Response Relationship, Drug,
pubmed-meshheading:17689858-Enzyme Activation,
pubmed-meshheading:17689858-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:17689858-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:17689858-Genistein,
pubmed-meshheading:17689858-Humans,
pubmed-meshheading:17689858-Mitochondria,
pubmed-meshheading:17689858-Models, Biological,
pubmed-meshheading:17689858-Receptors, TNF-Related Apoptosis-Inducing Ligand,
pubmed-meshheading:17689858-Stomach Neoplasms,
pubmed-meshheading:17689858-TNF-Related Apoptosis-Inducing Ligand
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pubmed:year |
2007
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pubmed:articleTitle |
Genistein sensitizes TRAIL-resistant human gastric adenocarcinoma AGS cells through activation of caspase-3.
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pubmed:affiliation |
Department of Biological Sciences, Pusan National University, Busan 609-735, Republic of Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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