|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1-3
|
|
pubmed:dateCreated |
1992-2-20
|
|
pubmed:abstractText |
A metallothionein cDNA was generated from pea (Pisum sativum L.) roots, amplified by PCR and inserted into a plasmid for expression in E. coli. Purification of the resultant product generated 3 pools of cadmium-containing material after DEAE-cellulose chromatography. The amino acid composition of each was in excellent agreement with that predicted for pea metallothionein. A cadmium content of approximately 6 g.atoms per mole of protein was estimated. N-terminal sequence analysis revealed that the recombinant molecule had been proteolysed within the extended region linking the 2 cysteine-rich (putative) metal-binding regions. The significance of these findings in terms of the protein folding/targeting of the molecule are considered.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Dec
|
|
pubmed:issn |
0014-5793
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
16
|
|
pubmed:volume |
295
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
171-5
|
|
pubmed:dateRevised |
2007-11-14
|
|
pubmed:meshHeading |
pubmed-meshheading:1765150-Amino Acid Sequence,
pubmed-meshheading:1765150-Base Sequence,
pubmed-meshheading:1765150-Chromatography, Gel,
pubmed-meshheading:1765150-Chromatography, High Pressure Liquid,
pubmed-meshheading:1765150-Chromatography, Ion Exchange,
pubmed-meshheading:1765150-Cloning, Molecular,
pubmed-meshheading:1765150-DNA,
pubmed-meshheading:1765150-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:1765150-Escherichia coli,
pubmed-meshheading:1765150-Fabaceae,
pubmed-meshheading:1765150-Metallothionein,
pubmed-meshheading:1765150-Molecular Sequence Data,
pubmed-meshheading:1765150-Plants, Medicinal,
pubmed-meshheading:1765150-Plasmids,
pubmed-meshheading:1765150-Polymerase Chain Reaction,
pubmed-meshheading:1765150-Protein Conformation,
pubmed-meshheading:1765150-Recombinant Proteins
|
|
pubmed:year |
1991
|
|
pubmed:articleTitle |
A plant metallothionein produced in E. coli.
|
|
pubmed:affiliation |
Department of Biochemistry, University of Wales College of Cardiff, UK.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|
