Linked Life Data

17618723

Source:http://linkedlifedata.com/resource/pubmed/id/17618723

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2007-7-30
pubmed:abstractText
The study highlights the adverse effects of organophosphate compounds dichlorvos and chlorpyrifos on reproduction in Drosophila. Freshly eclosed first instar larvae of Drosophila melanogaster transgenic for hsp70 (hsp70-lacZ) Bg(9) were fed on 0.015-150.0ppb dichlorvos and chlorpyrifos mixed food. Virgin flies eclosing from the normal and contaminated food were pair-mated to examine the effect of the test chemicals on reproduction of the exposed organisms. Expression of hsp70, sex peptide (SP or Acp70A), accessory gland protein (Acp36DE) and tissue damage was examined in reproductive organs of adult fly. Exposed organisms exhibited a dose-dependent significantly reduced reproductive outcome and males were found to be more sensitive than females. Hsp70 expression was restricted only within the testis lobes of male fly while it was not induced in the ovary of the female. In concurrence with absence of hsp70 expression in the accessory glands of male fly, tissue damage was evident in them. Acp70A and Acp36DE expression were found to be significantly downregulated at the higher concentrations of the test chemicals. The study suggests that (i) dichlorvos is more deleterious to fly reproduction compared to chlorpyrifos with an adverse effect on Acp70A and Acp36DE expression required to facilitate normal reproduction; (ii) hsp70 may be used as a marker of cellular damage against dichlorvos and chlorpyrifos in Drosophila.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0300-483X
pubmed:author
pubmed:issnType
Print
pubmed:day
16
pubmed:volume
238
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1-14
pubmed:dateRevised
2008-2-14
pubmed:meshHeading
pubmed-meshheading:17618723-Animals, pubmed-meshheading:17618723-Animals, Genetically Modified, pubmed-meshheading:17618723-Chlorpyrifos, pubmed-meshheading:17618723-Cholinesterase Inhibitors, pubmed-meshheading:17618723-Dichlorvos, pubmed-meshheading:17618723-Dose-Response Relationship, Drug, pubmed-meshheading:17618723-Drosophila Proteins, pubmed-meshheading:17618723-Drosophila melanogaster, pubmed-meshheading:17618723-Female, pubmed-meshheading:17618723-Gene Expression Regulation, Developmental, pubmed-meshheading:17618723-Genitalia, Male, pubmed-meshheading:17618723-HSP70 Heat-Shock Proteins, pubmed-meshheading:17618723-Immunohistochemistry, pubmed-meshheading:17618723-Larva, pubmed-meshheading:17618723-Male, pubmed-meshheading:17618723-Oviposition, pubmed-meshheading:17618723-Peptides, pubmed-meshheading:17618723-RNA, Messenger, pubmed-meshheading:17618723-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:17618723-Testis, pubmed-meshheading:17618723-beta-Galactosidase
pubmed:year
2007
pubmed:articleTitle
Adverse effect of organophosphate compounds, dichlorvos and chlorpyrifos in the reproductive tissues of transgenic Drosophila melanogaster: 70kDa heat shock protein as a marker of cellular damage.
pubmed:affiliation
Embryotoxicology Section, Industrial Toxicology Research Centre, Lucknow 226001, India.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't