Linked Life Data

1755920

Source:http://linkedlifedata.com/resource/pubmed/id/1755920

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1992-2-4
pubmed:abstractText
A sensitive sandwich enzyme-linked immunosorbent assay (ELISA) for specific detection of prototype cholera toxin (CT) elaborated by Vibrio cholerae serovar O1 has been developed. The use of a high affinity monoclonal antibody (MAb) for capturing of CT epitopes permitted a high efficiency. Using this ELISA, we sought in vitro production of CT from clinical strains of V. cholerae O1, Non-O1 and from LT-producing E. coli. All culture supernatants of V. cholerae O1 were positive for CT whereas V. cholerae non-O1 and LT producing E. coli were found negative for CT. This ELISA will be particularly useful in specifically designed studies where detection of CT and not of related labile toxins is mandatory.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0934-8840
pubmed:author
pubmed:issnType
Print
pubmed:volume
275
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
467-73
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
A polyclonal-monoclonal antibody based sensitive sandwich enzyme linked immunosorbent assay for specific detection of cholera toxin.
pubmed:affiliation
National Institute of Cholera and Enteric Diseases, Beliaghata, Calcutta, India.
pubmed:publicationType
Journal Article