Linked Life Data

17534407

Source:http://linkedlifedata.com/resource/pubmed/id/17534407

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2007-5-30
pubmed:abstractText
Human metapneumovirus and respiratory syncytial virus are RNA viruses associated with lower respiratory tract infections. Regular symptomatic re-infection and sequelae are common, particularly in individuals with pre-existing respiratory diseases, such as asthma. Our understanding of virus-dependent cytokine responses and potential differences between allergic asthmatics and non-asthmatics is limited. To test our hypothesis that adults with mild allergic asthma, the most common form of this disease, exhibit distinct pro-inflammatory responses, we developed a model using acute in vitro infection of fresh peripheral blood mononuclear cells. For both viruses, the production of innate-immunity-associated IL-6 and IL-10 was indistinguishable in the 2 populations. Type 1 cytokine production dominated adaptive immune responses in both asthmatic and non-asthmatic individuals. Surprisingly, asthmatics exhibited stronger pro-inflammatory IFNgamma production in response to human metapneumovirus than non-asthmatic adults (p = 0.01), with a similar, but statistically nonsignificant trend in the respiratory-syncytial-virus-stimulated response. Neutralizing IL-10 did not enhance the intensity of IFNgamma responses, demonstrating that this pro-inflammatory bias is not counter-regulated by IL-10. Finally, anti-TLR4 blocked lipopolysaccharide, but not respiratory-syncytial-virus-driven cytokine production. Collectively, the data demonstrate that asthma is characterized by markedly stronger pro-inflammatory IFNgamma responses to pneumoviruses than their non-asthmatic counterparts. This distinctive pattern of viral immunity may contribute to a worsening of asthma symptoms during respiratory virus infections.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0829-8211
pubmed:author
pubmed:issnType
Print
pubmed:volume
85
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
252-8
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:17534407-Adolescent, pubmed-meshheading:17534407-Adult, pubmed-meshheading:17534407-Antibodies, Monoclonal, pubmed-meshheading:17534407-Asthma, pubmed-meshheading:17534407-Cell Line, Tumor, pubmed-meshheading:17534407-Female, pubmed-meshheading:17534407-Humans, pubmed-meshheading:17534407-Immunity, Innate, pubmed-meshheading:17534407-Interferon-gamma, pubmed-meshheading:17534407-Interleukin-10, pubmed-meshheading:17534407-Interleukin-6, pubmed-meshheading:17534407-Lipopolysaccharides, pubmed-meshheading:17534407-Male, pubmed-meshheading:17534407-Metapneumovirus, pubmed-meshheading:17534407-Middle Aged, pubmed-meshheading:17534407-Respiratory Syncytial Virus Infections, pubmed-meshheading:17534407-Respiratory Syncytial Viruses, pubmed-meshheading:17534407-Toll-Like Receptor 4
pubmed:year
2007
pubmed:articleTitle
Adult asthmatics display exaggerated IFNgamma responses to human metapneumovirus and respiratory syncytial virus.
pubmed:affiliation
CIHR National Training Program in Allergy and Asthma Research, Department of Immunology, University of Manitoba, 603 Basic Medical Sciences Building, 730 William Ave, Winnipeg, MB R3E0W3, Canada.
pubmed:publicationType
Journal Article, Clinical Trial, Comparative Study, Research Support, Non-U.S. Gov't