Linked Life Data

1753412

Source:http://linkedlifedata.com/resource/pubmed/id/1753412

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1992-1-27
pubmed:abstractText
Mononuclear cells were isolated from a spleen excised during surgery and cultured with 0.05 KE (Klinische Einheit)/ml of the streptococcal preparation, OK-432, or 700 JRU (Japan Reference Units)/ml of recombinant interleukin-2 (rIL-2). When the cytotoxicity against K562 and Daudi cells was measured by four-hour 51Cr release assay, OK-432 induced about the same killing activity as that of rIL-2. The peak activity was observed on day 2. When the expression of human perforin messenger RNA (mRNA) was investigated by Northern blot analysis, a low-grade expression was seen in the splenocytes shortly after isolation (day 0). An expression 2.7-fold stronger than that seen on day 0 was produced after one day of culturing with OK-432. This enhanced expression decreased only slightly after the first day. The increases in expression of perforin mRNA on day 2 were similar for OK-432 and rIL-2. The results indicate the multicytokine inducer, OK-432, to induce perforin to about the same degree as rIL-2 does, and perforin to act as one of the effector molecules in the killing activity induced by OK-432.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0368-2811
pubmed:author
pubmed:issnType
Print
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
330-3
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
In vitro induction of perforin mRNA and cytotoxic activities in human splenocytes by the streptococcal preparation, OK-432.
pubmed:affiliation
First Department of Surgery, Okayama University Medical School.
pubmed:publicationType
Journal Article