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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
1992-1-24
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pubmed:abstractText |
Paroxysmal nocturnal haemoglobinuria (PNH) is now generally accepted as a disease in which bone marrow derived cells are deficient in phosphatidylinositolglycan (PIG)-anchored surface molecules. A series of new monoclonal antibodies detecting PIG-anchored surface structures on human leucocytes (CD48, CD55, CD59) has recently been described. In the present study 12 patients with the diagnosis PNH and a positive Ham test were examined for PIG-anchored surface antigen expression on various cell lineages using immunofluorescence. In all patients deficient cells were detected in erythrocyte, granulocyte and monocyte analysis. A deficient lymphocyte subset was also observed in all but one of these patients. Using two-colour analysis, all lymphocyte subpopulations such as T, B and NK cells were found to be affected. In addition, peripheral blood cells of 22 patients with severe aplastic anaemia (SAA) were tested for the PIG-anchoring defect. In five of these patients the defect was detected, and in four of the five the lack of PIG-anchored molecules was confined to the granulocyte and monocyte lineages apparently without affecting the erythrocytes. The results of these studies demonstrate that cytofluorographic testing of peripheral blood cells provides a simple and reliable method for establishing the diagnosis of PNH. Furthermore, especially in the case of aplastic anaemia patients, the sensitivity of immunophenotyping might be superior to conventional laboratory tests.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Glycosylphosphatidylinositols,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositols,
http://linkedlifedata.com/resource/pubmed/chemical/Polysaccharides
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0007-1048
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
79
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
487-92
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1751377-Anemia, Aplastic,
pubmed-meshheading:1751377-Antibodies, Monoclonal,
pubmed-meshheading:1751377-Antigens, CD,
pubmed-meshheading:1751377-Erythrocytes,
pubmed-meshheading:1751377-Glycosylphosphatidylinositols,
pubmed-meshheading:1751377-Granulocytes,
pubmed-meshheading:1751377-Hemoglobinuria, Paroxysmal,
pubmed-meshheading:1751377-Humans,
pubmed-meshheading:1751377-Lymphocytes,
pubmed-meshheading:1751377-Monocytes,
pubmed-meshheading:1751377-Phosphatidylinositols,
pubmed-meshheading:1751377-Polysaccharides
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pubmed:year |
1991
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pubmed:articleTitle |
Diagnosis of paroxysmal nocturnal haemoglobinuria using immunophenotyping of peripheral blood cells.
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pubmed:affiliation |
Abteilung Immunologie und Transfusionsmedizin, Medizinische Hochschule, Hannover, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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