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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
2007-5-3
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pubmed:abstractText |
In this study, we examine whether native cholera toxin (nCT) as a mucosal adjuvant can support trinitrophenyl (TNP)-LPS-specific mucosal immune responses. C57BL/6 mice were given nasal TNP-LPS in the presence or absence of nCT. Five days later, significantly higher levels of TNP-specific mucosal IgA Ab responses were induced in the nasal washes, saliva, and plasma of mice given nCT plus TNP-LPS than in those given TNP-LPS alone. High numbers of TNP-specific IgA Ab-forming cells were also detected in mucosal tissues such as the nasal passages (NPs), the submandibular glands (SMGs), and nasopharyngeal-associated lymphoreticular tissue of mice given nCT. Flow cytometric analysis showed that higher numbers of surface IgA+, CD5+ B cells (B-1a B cells) in SMGs and NPs of mice given nasal TNP-LPS plus nCT than in those given TNP-LPS alone. Furthermore, increased levels of IL-5R alpha-chain were expressed by B-1a B cells in SMGs and NPs of mice given nasal TNP-LPS plus nCT. Thus, CD4+ T cells from these mucosal effector lymphoid tissues produce high levels of IL-5 at both protein and mRNA levels. When mice were treated with anti-IL-5 mAb, significant reductions in TNP-specific mucosal IgA Ab responses were noted in external secretions. These findings show that nasal nCT as an adjuvant enhances mucosal immune responses to a T cell-independent Ag due to the cross-talk between IL-5Ralpha+ B-1a B cells and IL-5-producing CD4+ T cells in the mucosal effector lymphoid tissues.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adjuvants, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, T-Independent,
http://linkedlifedata.com/resource/pubmed/chemical/Cholera Toxin,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes, B-Lymphocyte,
http://linkedlifedata.com/resource/pubmed/chemical/Forkhead Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Foxb1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin A,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-5,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-5 Receptor alpha Subunit,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/trinitrophenyl-lipopolysaccharide
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0022-1767
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pubmed:author |
pubmed-author:FujihashiKeikoK,
pubmed-author:FujihashiKohtaroK,
pubmed-author:FukuiwaTatsuyaT,
pubmed-author:KataokaKosukeK,
pubmed-author:KobayashiRyokiR,
pubmed-author:McGheeJerry RJR,
pubmed-author:NagataHidekiH,
pubmed-author:SekineShinichiS,
pubmed-author:ShizukuishiSatoshiS,
pubmed-author:SuzukiHideakiH,
pubmed-author:TakatsuKiyoshiK
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
178
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6058-65
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:17475830-Adjuvants, Immunologic,
pubmed-meshheading:17475830-Animals,
pubmed-meshheading:17475830-Antigens, T-Independent,
pubmed-meshheading:17475830-B-Lymphocyte Subsets,
pubmed-meshheading:17475830-Cholera Toxin,
pubmed-meshheading:17475830-Epitopes, B-Lymphocyte,
pubmed-meshheading:17475830-Female,
pubmed-meshheading:17475830-Forkhead Transcription Factors,
pubmed-meshheading:17475830-Immunity, Innate,
pubmed-meshheading:17475830-Immunity, Mucosal,
pubmed-meshheading:17475830-Immunoglobulin A,
pubmed-meshheading:17475830-Interleukin-5,
pubmed-meshheading:17475830-Interleukin-5 Receptor alpha Subunit,
pubmed-meshheading:17475830-Lipopolysaccharides,
pubmed-meshheading:17475830-Mice,
pubmed-meshheading:17475830-Mice, Inbred C57BL,
pubmed-meshheading:17475830-Nasal Mucosa,
pubmed-meshheading:17475830-Submandibular Gland
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pubmed:year |
2007
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pubmed:articleTitle |
Nasal cholera toxin elicits IL-5 and IL-5 receptor alpha-chain expressing B-1a B cells for innate mucosal IgA antibody responses.
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pubmed:affiliation |
Department of Pediatric Dentistry, Immunobiology Vaccine Center, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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