Linked Life Data

17443671

Source:http://linkedlifedata.com/resource/pubmed/id/17443671

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2007-6-4
pubmed:abstractText
M-CSF is a cytokine essential for both the proliferation and differentiation of monocytes/macrophages. In this study, we established a new M-CSF-mediated differentiation-inducing system, and examined how the level and duration of the activation of ERK preceded M-CSF-mediated differentiation. TF-1-fms human leukemia cells rapidly proliferated in response to M-CSF. However, in the presence of a phorbol ester, TPA, TF-1-fms cells definitely switched their responsiveness to M-CSF from proliferation to differentiation, as evidenced by a more drastic morphological change and the appearance of cells with a higher level of phagocytic activity. In TF-1-fms cells expressing HIV-1 Nef protein in a conditionally active-manner, both M-CSF-mediated proliferation and M-CSF/TPA-mediated differentiation were inhibited by the activation of Nef. The Nef-active cells showed perturbed patterns of ERK activation. Under the proliferation-inducing conditions (TPA-free), parental or Nef-inactive cells showed modest ERK activation following M-CSF stimulation, whereas Nef-active cells showed an earlier and transient ERK activation, despite a decrease in their proliferation rate. Under the differentiation-inducing conditions, parental or Nef-inactive cells showed increased and prolonged ERK activation following M-CSF stimulation, whereas Nef-active cells showed transient ERK activation. These results supported the idea that the increased and prolonged ERK activation led to M-CSF-mediated macrophage differentiation but not to proliferation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0021-9541
pubmed:author
pubmed:issnType
Print
pubmed:volume
212
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
519-25
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:17443671-Cell Differentiation, pubmed-meshheading:17443671-Cell Line, Tumor, pubmed-meshheading:17443671-Cell Proliferation, pubmed-meshheading:17443671-Cell Shape, pubmed-meshheading:17443671-Dose-Response Relationship, Drug, pubmed-meshheading:17443671-Enzyme Activation, pubmed-meshheading:17443671-Extracellular Signal-Regulated MAP Kinases, pubmed-meshheading:17443671-Gene Products, nef, pubmed-meshheading:17443671-Humans, pubmed-meshheading:17443671-Macrophage Colony-Stimulating Factor, pubmed-meshheading:17443671-Macrophages, pubmed-meshheading:17443671-Phagocytosis, pubmed-meshheading:17443671-Tetradecanoylphorbol Acetate, pubmed-meshheading:17443671-Time Factors, pubmed-meshheading:17443671-Transfection, pubmed-meshheading:17443671-nef Gene Products, Human Immunodeficiency Virus
pubmed:year
2007
pubmed:articleTitle
M-CSF-mediated macrophage differentiation but not proliferation is correlated with increased and prolonged ERK activation.
pubmed:affiliation
Division of Hematopoiesis, Center for AIDS Research, Kumamoto University, Kumamoto, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't