Linked Life Data

1744102

Source:http://linkedlifedata.com/resource/pubmed/id/1744102

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
34
pubmed:dateCreated
1992-1-13
pubmed:databankReference
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M55199, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M55200, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M55201, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M74491, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M74492, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M74992, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M74993, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M96681, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M96682, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/S66610, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/S66768, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/S68759, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/S68761, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/S68763
pubmed:abstractText
ADP-ribosylation factors (ARFs) are approximately 20-kDa guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin in vitro. Five different human ARFs have been identified by cDNA cloning. Northern analysis using ARF 3-specific oligonucleotides identified two mRNAs of 3.7 and 1.2 kilobases (kb). We report here the complete nucleotide sequence of the 3.7-kb ARF 3 mRNA derived from three overlapping cDNAs isolated from human hippocampus and fetal brain cDNA libraries, as well as the structure of human ARF 3 gene. Sequences of two overlapping genomic clones indicated that the ARF 3 gene spans approximately 18.3 kb and contains five exons and four introns. The conserved amino acid sequences involved in guanine nucleotide binding by ARF 3 are distributed among separate exons, as found in other GTP-binding protein genes. Translation initiates in exon 2 which includes the sequence GXXXXGK that probably participates in phosphate binding and GTP hydrolysis. The sequence DVGG in exon 3 coordinates binding of Mg2+ and the beta-phosphate of GDP. In the ARF 3 gene in contrast to those of other GTP-binding proteins, the sequence NKXD (which is thought to contribute to the specificity of interaction with the guanine ring) is divided between exons 4 and 5. The latter encodes the COOH-terminal 53 amino acids of ARF 3 and contains greater than 2500 base pairs of untranslated DNA. The sequence AATTAA is 19 bases 5' to the polyadenylation addition site of the 3.7-kb mRNA. Multiple transcription start sites were identified by primer extension and S1 and mung bean nuclease analyses. The 5'-flanking region of exon 1 contains neither a TATA nor a CAAT box, but is high in GC content (greater than 70%) and includes three potential Sp1-binding sites (GC box), consistent with the promoters described for several housekeeping genes. The 1.2-kb ARF 3 mRNA is shown to arise by use of an alternative polyadenylation signal (AACAAA) at nucleotide 1091 within the ARF 3 cDNA.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
5
pubmed:volume
266
pubmed:geneSymbol
ARF 3
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
23053-9
pubmed:dateRevised
2005-11-17
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Isolation and characterization of the human gene for ADP-ribosylation factor 3, a 20-kDa guanine nucleotide-binding protein activator of cholera toxin.
pubmed:affiliation
Laboratory of Cellular Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892.
pubmed:publicationType
Journal Article