Linked Life Data

17403913

Source:http://linkedlifedata.com/resource/pubmed/id/17403913

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2007-6-20
pubmed:abstractText
Arylamine N-acetyltransferases (NAT) catalyze the biotransformation of many important arylamine drugs and procarcinogens. NAT can either detoxify or activate procarcinogens, complicating the manner in which these enzymes may participate in enhancing or preventing toxic responses to particular agents. Mice possess three NAT isoenzymes: Nat1, Nat2, and Nat3. Whereas Nat1 and Nat2 can efficiently acetylate many arylamines, few substrates appear to be appreciably metabolized by Nat3. We generated a Nat3 knockout mouse strain and used it along with our double Nat1/2(-/-) knockout strain to further investigate the functional role of Nat3. Nat3(-/-) mice showed normal viability and reproductive capacity. Nat3 expression was very low in wild-type animals and completely undetectable in Nat3(-/-) mice. In contrast, greatly elevated expression of Nat3 transcript was observed in Nat1/2(-/-) mice. We used a transcribed marker polymorphism approach to establish that the increased expression of Nat3 in Nat1/2(-/-) mice is a positional artifact of insertion of the phosphoglycerate kinase-neomycin resistance cassette in place of the Nat1/Nat2 gene region and upstream of the intact Nat3 gene, rather than a biological compensatory mechanism. Despite the increase in Nat3 transcript, the N-acetylation of p-aminosalicylate, sulfamethazine, 2-aminofluorene, and 4-aminobiphenyl was undetectable either in vivo or in vitro in Nat1/2(-/-) animals. In parallel, no difference was observed in the in vivo clearance or in vitro metabolism of any of these substrates between wild-type and Nat3(-/-) mice. Thus, Nat3 is unlikely to play a significant role in the N-acetylation of arylamines either in wild-type mice or in mice lacking Nat1 and Nat2 activities.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0090-9556
pubmed:author
pubmed:issnType
Print
pubmed:volume
35
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1064-70
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:17403913-Acetylation, pubmed-meshheading:17403913-Aminobiphenyl Compounds, pubmed-meshheading:17403913-Aminosalicylic Acid, pubmed-meshheading:17403913-Animals, pubmed-meshheading:17403913-Artifacts, pubmed-meshheading:17403913-Arylamine N-Acetyltransferase, pubmed-meshheading:17403913-Female, pubmed-meshheading:17403913-Fluorenes, pubmed-meshheading:17403913-Gene Expression Regulation, Enzymologic, pubmed-meshheading:17403913-Isoenzymes, pubmed-meshheading:17403913-Male, pubmed-meshheading:17403913-Mice, pubmed-meshheading:17403913-Mice, Inbred C57BL, pubmed-meshheading:17403913-Mice, Knockout, pubmed-meshheading:17403913-RNA, Messenger, pubmed-meshheading:17403913-Sex Factors, pubmed-meshheading:17403913-Substrate Specificity, pubmed-meshheading:17403913-Sulfamethazine
pubmed:year
2007
pubmed:articleTitle
Effect of arylamine acetyltransferase Nat3 gene knockout on N-acetylation in the mouse.
pubmed:affiliation
Department of Pharmacology, University of Toronto, Toronto, ON, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't