Linked Life Data

1733065

Source:http://linkedlifedata.com/resource/pubmed/id/1733065

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1992-2-24
pubmed:abstractText
The purpose of the present study was to investigate the phenotype of macrophages that infiltrate normal and transplanted rat tissues. The macrophage monoclonal antibodies ED1, ED2, ED3, 52-1D4, ER15, and OX43, together with antibodies against lymphocyte and class II MHC antigens, were used in an indirect immunofluorescence technique with sections of normal tissues and heart and renal grafts that experienced long-term survival or rejection. A small number of ED1- and ED3-positive interstitial cells were detected in normal heart and renal tissues and their number increased dramatically in rejection. Normal heart tissue contained a population of ED2-positive cells with dendritic morphology that was not detected in renal tissue. Following transplantation, a diffuse increase of rounded ED2-positive cells was observed in heart grafts; no ED2-positive cells were detected in grafts removed after 20-30 days from nonimmunosuppressed recipients. Grafts from CsA-treated animals or grafts that survived greater than 50 days in nonimmunosuppressed recipients exhibited the interstitial dendritic pattern of ED2-positive cells. Only very few rounded ED2-positive cells were observed in renal allografts; if present, they were mostly located in the medulla. OX43, which bound in normal tissues to vessel endothelium and a population of macrophages, stained in allografts an additional small population of graft-infiltrating cells, and in F344 renal allografts a population of multinucleated giant cells. We conclude that the posttransplant macrophage infiltration pattern of heart and renal allografts, defined by the monocyte/macrophage antibodies ED1, ED3, 52-1D4, and ER15, is very similar for both types of organs, although the antibody ED2 and the endothelial-macrophage antibody OX43 revealed remarkable differences between the two types of organ allografts.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0041-1337
pubmed:author
pubmed:issnType
Print
pubmed:volume
53
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
157-62
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Macrophage subpopulations in normal and transplanted heart and kidney tissues in the rat.
pubmed:affiliation
Department of Medicine, University of Calgary, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't