Linked Life Data

17313953

Source:http://linkedlifedata.com/resource/pubmed/id/17313953

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2007-3-19
pubmed:abstractText
A large number of analytes, including non-fluorescent ones, can be sensitively detected by fluorescence scanning densitometry using silica gel HPTLC plates impregnated with a solution of coralyne cation. This is carried out by the variation, increase or decrease, that the corresponding analyte induces on native coralyne emission at a given excitation wavelength. A similar phenomenon was previously described for berberine cation, and Reichardt's dye probes. However, the sensitivity of coralyne in HPTLC detection of non-fluorescent, structurally different analytes (e.g., long-chain alkanes, alcohols, alkylbromides, neutral lipids) is superior to that of the above-mentioned probes. In this work, the analytical viability of this phenomenon for HPTLC detection using coralyne as a probe is explored, and fluorescent responses of a number of analytes on the coralyne system are rationalized in the light of a previously proposed model. This establishes that the resulting intensity for a probe in the presence of a given compound can be explained as a balance between radiative (contribution of non-specific interactions) and non-radiative processes (specific interactions), the latter producing fluorescence quenching. Experimental results and proposed model suggest that this phenomenon may be general for practically all kinds of analytes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0021-9673
pubmed:author
pubmed:issnType
Print
pubmed:day
6
pubmed:volume
1146
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
251-7
pubmed:dateRevised
2009-1-15
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Coralyne cation, a fluorescent probe for general detection in planar chromatography.
pubmed:affiliation
Instituto de Carboquímica, CSIC, P.O. Box 549, 50080 Zaragoza, Spain.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't