Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2007-3-29
pubmed:abstractText
Genetic analysis of the Drosophila Bloom's syndrome helicase homolog (mus309/DmBLM) indicates that DmBLM is required for the synthesis-dependent strand annealing (SDSA) pathway of homologous recombination. Here we report the first biochemical study of DmBLM. Recombinant, epitope-tagged DmBLM was expressed in Drosophila cell culture and highly purified protein was prepared from nuclear extracts. Purified DmBLM exists exclusively as a high molecular weight ( approximately 1.17 MDa) species, is a DNA-dependent ATPase, has 3'-->5' DNA helicase activity, prefers forked substrate DNAs and anneals complementary DNAs. High-affinity DNA binding is ATP-dependent and low-affinity ATP-independent interactions contribute to forked substrate DNA binding and drive strand annealing. DmBLM combines DNA strand displacement with DNA strand annealing to catalyze the displacement of one DNA strand while annealing a second complementary DNA strand.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/17272294-10049920, http://linkedlifedata.com/resource/pubmed/commentcorrection/17272294-10319867, http://linkedlifedata.com/resource/pubmed/commentcorrection/17272294-10359700, http://linkedlifedata.com/resource/pubmed/commentcorrection/17272294-10734115, http://linkedlifedata.com/resource/pubmed/commentcorrection/17272294-10835635, http://linkedlifedata.com/resource/pubmed/commentcorrection/17272294-11278509, http://linkedlifedata.com/resource/pubmed/commentcorrection/17272294-11283371, http://linkedlifedata.com/resource/pubmed/commentcorrection/17272294-11309417, http://linkedlifedata.com/resource/pubmed/commentcorrection/17272294-11433031, http://linkedlifedata.com/resource/pubmed/commentcorrection/17272294-12072467, http://linkedlifedata.com/resource/pubmed/commentcorrection/17272294-12522255, http://linkedlifedata.com/resource/pubmed/commentcorrection/17272294-12803543, http://linkedlifedata.com/resource/pubmed/commentcorrection/17272294-14622595, http://linkedlifedata.com/resource/pubmed/commentcorrection/17272294-14685245, http://linkedlifedata.com/resource/pubmed/commentcorrection/17272294-15065659, http://linkedlifedata.com/resource/pubmed/commentcorrection/17272294-15184650, http://linkedlifedata.com/resource/pubmed/commentcorrection/17272294-15238522, http://linkedlifedata.com/resource/pubmed/commentcorrection/17272294-15241474, http://linkedlifedata.com/resource/pubmed/commentcorrection/17272294-15501916, http://linkedlifedata.com/resource/pubmed/commentcorrection/17272294-15775963, http://linkedlifedata.com/resource/pubmed/commentcorrection/17272294-15845538, http://linkedlifedata.com/resource/pubmed/commentcorrection/17272294-15899892, http://linkedlifedata.com/resource/pubmed/commentcorrection/17272294-15990871, http://linkedlifedata.com/resource/pubmed/commentcorrection/17272294-16024743, http://linkedlifedata.com/resource/pubmed/commentcorrection/17272294-16412221, http://linkedlifedata.com/resource/pubmed/commentcorrection/17272294-16537486, http://linkedlifedata.com/resource/pubmed/commentcorrection/17272294-16670433, http://linkedlifedata.com/resource/pubmed/commentcorrection/17272294-16705162, http://linkedlifedata.com/resource/pubmed/commentcorrection/17272294-4140506, http://linkedlifedata.com/resource/pubmed/commentcorrection/17272294-6795083, http://linkedlifedata.com/resource/pubmed/commentcorrection/17272294-7585968, http://linkedlifedata.com/resource/pubmed/commentcorrection/17272294-8602509, http://linkedlifedata.com/resource/pubmed/commentcorrection/17272294-8608940, http://linkedlifedata.com/resource/pubmed/commentcorrection/17272294-8913739, http://linkedlifedata.com/resource/pubmed/commentcorrection/17272294-9388193
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1362-4962
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
35
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1367-76
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
DNA strand displacement, strand annealing and strand swapping by the Drosophila Bloom's syndrome helicase.
pubmed:affiliation
Department of Molecular and Cell Biology, 16 Barker Hall #3204, University of California Berkeley, CA 94720-3204, USA.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural