Linked Life Data

17239897

Source:http://linkedlifedata.com/resource/pubmed/id/17239897

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2007-2-12
pubmed:abstractText
A new method for the enrichment of Strychnos alkaloids in biological samples via liquid-phase microextraction (LPME) based on porous polypropylene hollow fibers combined with on-line sweeping in micellar electrokinetic chromatography (MEKC) was developed. Strychnos alkaloids were first extracted from urine sample which was adjusted to alkaline conditions (0.5 mol l(-1) NaOH). The unionized analytes were subsequently extracted into 1-octanol impregnated in the pores of hollow fibers, and then into an acidic acceptor solution (100 mmol l(-1) H3PO4) inside the hollow fiber. The extract was analyzed directly by on-line sweeping in MEKC. In the method, the compound berberine was used as the internal standard (I.S.) for the improvement of the experimental reproducibility. The calibration curve was linear over a range of 20-200 ng ml(-1) for both strychnine and brucine in human urine sample, with a correlation coefficient of 0.996 and 0.997, respectively. The detection limits (S/N=3:1) for strychnine and brucine were 1 and 2 ng ml(-1), respectively. The LPME-sweeping method has been successfully applied to the analysis of strychnine and brucine in real urine sample, indicating that LPME-sweeping-MEKC is a promising combination for analysis of basic drugs present at low levels in some biological matrices.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0021-9673
pubmed:author
pubmed:issnType
Print
pubmed:day
2
pubmed:volume
1143
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
270-5
pubmed:dateRevised
2009-1-15
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Hollow fiber-based liquid-phase microextraction combined with on-line sweeping for trace analysis of Strychnos alkaloids in urine by micellar electrokinetic chromatography.
pubmed:affiliation
Key Laboratory of Bioinorganic Chemistry, College of Science, Agricultural University of Hebei, Baoding 071001, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't