Linked Life Data

17227720

Source:http://linkedlifedata.com/resource/pubmed/id/17227720

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2007-4-30
pubmed:abstractText
A comparison between HPLC with conventional fluorescence detection and capillary-LC (microHPLC) with native laser-induced fluorescence (LIF) detection was done to determine chloroquine (CQ) and quinine (Q) in human serum. HPLC experiments were run with parameters of the conventional fluorimeter set at the highest level of sensitivity. Results were compared with those obtained on microHPLC coupled to a ZETALIF (He-Cd 325 nm) detector which provided a 50-fold increase in sensitivity. In microHPLC-LIF injection volumes were 200 nL instead of 10 microL in conventional HPLC. The separation was completed within 3 min (6 min on HPLC). The limit of detection on microHPLC-LIF was 1.9 and 1.3 fmol for CQ and Q, respectively. Both experiments were validated on serum samples. The mean recovery was more than 95% for CQ and Q. The intra- and inter-day precision and accuracy were found to be within the acceptable limits (<10%).
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1570-0232
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
850
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
481-7
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Very small injected samples to study chloroquine and quinine in human serum using capillary-LC and native fluorescence.
pubmed:affiliation
UMR 152-IRD-Université Paul Sabatier, Toulouse 3, 31062 Toulouse Cedex 9, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't