Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2007-3-9
pubmed:abstractText
The yeast Saccharomyces cerevisiae utilizes rapidly responding mitogen-activated protein kinase (MAPK) signaling cascades to adapt efficiently to a changing environment. Here we report that phosphorylation of Cdc37p, an Hsp90 cochaperone, by casein kinase 2 controls the functionality of two MAPK cascades in yeast. These pathways, the high-osmolarity glycerol (HOG) pathway and the cell integrity (protein kinase C) MAPK pathway, mediate adaptive responses to high osmotic and cell wall stresses, respectively. Mutation of the phosphorylation site Ser14 in Cdc37p renders cells sensitive to osmotic stress and cell wall perturbation by calcofluor white. We found that levels of the MAPKs Hog1p and Slt2p (Mpk1p) in cells are reduced in a cdc37-S14A mutant, and consequently downstream responses mediated by Hog1p and Slt2p are compromised. Furthermore, we present evidence that Hog1p and Slt2p both interact in a complex with Cdc37p in vivo, something that has not been reported previously. The interaction of Hsp90, Slt2p, and Hog1p with Cdc37p depends on the phosphorylation status of Cdc37p. In fact, our biochemical data show that the osmosensitive phenotype of the cdc37-S14A mutant is due to the loss of the interaction between Cdc37p, Hog1p, and Hsp90. Likewise, during cell wall stress, the interaction of Slt2p with Cdc37p and Hsp90 is crucial for Slt2p-dependent downstream responses, such as the activation of the transcription factor Rlm1p. Interestingly, phosphorylated Slt2p, but not phosphorylated Hog1p, has an increased affinity for Cdc37p. Together these observations suggest that Cdc37p acts as a regulator of MAPK signaling.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-10022854, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-10348843, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-10594829, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-10629030, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-10664467, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-10858314, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-11036079, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-11085988, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-11206552, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-11413142, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-11916974, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-11988487, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-12040128, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-12176997, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-12410835, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-12435747, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-12482976, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-12499358, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-12724381, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-12823808, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-12828945, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-12861001, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-12930845, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-1331109, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-1386897, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-14580204, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-14627196, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-15033927, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-15082798, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-15853661, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-15879519, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-16098195, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-16487343, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-16551624, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-16949366, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-16950928, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-1897313, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-3897187, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-7002718, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-7565726, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-7592778, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-7753858, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-7760835, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-7783209, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-7939632, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-8020093, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-8085158, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-8389377, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-8530423, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-8666233, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-8703009, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-8885235, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-8890173, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-9060463, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-9065400, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-9111331, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-9132011, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-9242486, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-9305635, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-9398671, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-9665134, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-9685350, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-9717241, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-9841672, http://linkedlifedata.com/resource/pubmed/commentcorrection/17220467-9843569
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/CDC37 protein, S cerevisiae, http://linkedlifedata.com/resource/pubmed/chemical/Cell Cycle Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Glycerol, http://linkedlifedata.com/resource/pubmed/chemical/HOG1 protein, S cerevisiae, http://linkedlifedata.com/resource/pubmed/chemical/HSP90 Heat-Shock Proteins, http://linkedlifedata.com/resource/pubmed/chemical/MADS Domain Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases, http://linkedlifedata.com/resource/pubmed/chemical/Molecular Chaperones, http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C, http://linkedlifedata.com/resource/pubmed/chemical/RLM1 protein, S cerevisiae, http://linkedlifedata.com/resource/pubmed/chemical/SLT2 protein, S cerevisiae, http://linkedlifedata.com/resource/pubmed/chemical/Saccharomyces cerevisiae Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1535-9778
pubmed:author
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