Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1976-1-23
pubmed:abstractText
Survival and reversion to T4r+ of bacteriophage T4rII AP72 after treatment with ethyl methanesulphonate at 37 degrees or 45 degrees C were studied in relation to the extent and mode of alkylation of purines in DNA of ethylated bacteriophage. A single-burst technique was used for reversion assay. Survival was lower at 45 degrees C than at 37 degrees C at a given extent of ethylation of bacteriophage DNA, confirming that events subsequent to ethylation, probably depurinations, are the main cause of decreased survival. Reversion was positively correlated (approximately linearly except at low extents at 37 degrees C) with ethylation of bacteriophage DNA, showing that ethylation itself causes mutation. Following the concept that reversion results from G-C leads to A-T transition at a single site (Krieg, 1963a,b) and the suggestion that O6-alkylation of guanine generates the miscoding base (Loveless, 1969), it was calculated that about one-third of induced O6-ethylguanines at this site would miscode to induce mutation.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/172067-13063483, http://linkedlifedata.com/resource/pubmed/commentcorrection/172067-14035786, http://linkedlifedata.com/resource/pubmed/commentcorrection/172067-14100794, http://linkedlifedata.com/resource/pubmed/commentcorrection/172067-172066, http://linkedlifedata.com/resource/pubmed/commentcorrection/172067-4590108, http://linkedlifedata.com/resource/pubmed/commentcorrection/172067-4673570, http://linkedlifedata.com/resource/pubmed/commentcorrection/172067-4923669, http://linkedlifedata.com/resource/pubmed/commentcorrection/172067-4945317, http://linkedlifedata.com/resource/pubmed/commentcorrection/172067-5269245, http://linkedlifedata.com/resource/pubmed/commentcorrection/172067-5337700, http://linkedlifedata.com/resource/pubmed/commentcorrection/172067-5435496, http://linkedlifedata.com/resource/pubmed/commentcorrection/172067-5791738, http://linkedlifedata.com/resource/pubmed/commentcorrection/172067-5816575
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0264-6021
pubmed:author
pubmed:issnType
Print
pubmed:volume
145
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
85-91
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1975
pubmed:articleTitle
Molecular mechanisms in alkylation mutagenesis. Induced reversion of bacteriophage T4rII AP72 by ethyl methanesulphonate in relation to extent and mode of ethylation of purines in bacteriophage deoxyribonucleic acid.
pubmed:publicationType
Journal Article