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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1991-11-1
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pubmed:abstractText |
We describe a rabbit intralymphnode immunization technique for obtaining a high titer antihuman CSF-1 antiserum with small amounts of antigen. This procedure provided a rapid (42 days after primo-injection), stable maximum immune response with a high titer antiserum precipitating 30% of 125I CSF-1 at a 1:25.000 dilution. The specificity of the immune serum was assessed by competitive binding experiments in RIA and neutralization of the CSF-1 biological activity in culture. The antiserum was also tested for its ability to detect CSF-1 in Western blotting, immunocytochemistry and immunohisto-chemistry. The results show that the immune serum specifically recognizes the biological active domain of human CSF-1 molecules from different origins and only detects dimeric forms. The potential uses of this anti CSF-1 antiserum are discussed.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1148-5493
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
1
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
177-83
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1717026-Animals,
pubmed-meshheading:1717026-Antibodies,
pubmed-meshheading:1717026-Antibody Specificity,
pubmed-meshheading:1717026-Binding, Competitive,
pubmed-meshheading:1717026-Blotting, Western,
pubmed-meshheading:1717026-Cell Line,
pubmed-meshheading:1717026-Colony-Forming Units Assay,
pubmed-meshheading:1717026-Epitopes,
pubmed-meshheading:1717026-Humans,
pubmed-meshheading:1717026-Immune Sera,
pubmed-meshheading:1717026-Immunization,
pubmed-meshheading:1717026-Immunoenzyme Techniques,
pubmed-meshheading:1717026-Injections, Intralymphatic,
pubmed-meshheading:1717026-Lymph Nodes,
pubmed-meshheading:1717026-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:1717026-Male,
pubmed-meshheading:1717026-Neutralization Tests,
pubmed-meshheading:1717026-Rabbits,
pubmed-meshheading:1717026-Radioimmunoassay,
pubmed-meshheading:1717026-Recombinant Proteins
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pubmed:articleTitle |
Intralymphnode injection of human monocyte macrophage colony stimulating factor (CSF-1) as a method of obtaining high titer anti-CSF-1 antibodies.
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pubmed:affiliation |
Laboratoire d'Hematologie, Centre Hospitalier Regional Universitaire, Nantes, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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