Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2007-2-7
pubmed:abstractText
We have established a reverse genetics approach for the routine generation of medaka (Oryzias latipes) gene knockouts. A cryopreserved library of N-ethyl-N-nitrosourea (ENU) mutagenized fish was screened by high-throughput resequencing for induced point mutations. Nonsense and splice site mutations were retrieved for the Blm, Sirt1, Parkin and p53 genes and functional characterization of p53 mutants indicated a complete knockout of p53 function. The current cryopreserved resource is expected to contain knockouts for most medaka genes.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/17156454-10588737, http://linkedlifedata.com/resource/pubmed/commentcorrection/17156454-10588859, http://linkedlifedata.com/resource/pubmed/commentcorrection/17156454-10748531, http://linkedlifedata.com/resource/pubmed/commentcorrection/17156454-11017081, http://linkedlifedata.com/resource/pubmed/commentcorrection/17156454-11250074, http://linkedlifedata.com/resource/pubmed/commentcorrection/17156454-11580890, http://linkedlifedata.com/resource/pubmed/commentcorrection/17156454-11823791, http://linkedlifedata.com/resource/pubmed/commentcorrection/17156454-12098699, http://linkedlifedata.com/resource/pubmed/commentcorrection/17156454-12116161, http://linkedlifedata.com/resource/pubmed/commentcorrection/17156454-12574629, http://linkedlifedata.com/resource/pubmed/commentcorrection/17156454-12597435, http://linkedlifedata.com/resource/pubmed/commentcorrection/17156454-14534297, http://linkedlifedata.com/resource/pubmed/commentcorrection/17156454-14613981, http://linkedlifedata.com/resource/pubmed/commentcorrection/17156454-15040442, http://linkedlifedata.com/resource/pubmed/commentcorrection/17156454-15210172, http://linkedlifedata.com/resource/pubmed/commentcorrection/17156454-15210174, http://linkedlifedata.com/resource/pubmed/commentcorrection/17156454-15496914, http://linkedlifedata.com/resource/pubmed/commentcorrection/17156454-1552940, http://linkedlifedata.com/resource/pubmed/commentcorrection/17156454-15630097, http://linkedlifedata.com/resource/pubmed/commentcorrection/17156454-15694309, http://linkedlifedata.com/resource/pubmed/commentcorrection/17156454-1596275, http://linkedlifedata.com/resource/pubmed/commentcorrection/17156454-16439994, http://linkedlifedata.com/resource/pubmed/commentcorrection/17156454-16475007, http://linkedlifedata.com/resource/pubmed/commentcorrection/17156454-16493422, http://linkedlifedata.com/resource/pubmed/commentcorrection/17156454-16495775, http://linkedlifedata.com/resource/pubmed/commentcorrection/17156454-2514168, http://linkedlifedata.com/resource/pubmed/commentcorrection/17156454-3409256, http://linkedlifedata.com/resource/pubmed/commentcorrection/17156454-7585968, http://linkedlifedata.com/resource/pubmed/commentcorrection/17156454-7651740, http://linkedlifedata.com/resource/pubmed/commentcorrection/17156454-8023157, http://linkedlifedata.com/resource/pubmed/commentcorrection/17156454-9007226, http://linkedlifedata.com/resource/pubmed/commentcorrection/17156454-9007227, http://linkedlifedata.com/resource/pubmed/commentcorrection/17156454-9161419, http://linkedlifedata.com/resource/pubmed/commentcorrection/17156454-9207020, http://linkedlifedata.com/resource/pubmed/commentcorrection/17156454-9582016, http://linkedlifedata.com/resource/pubmed/commentcorrection/17156454-9731209, http://linkedlifedata.com/resource/pubmed/commentcorrection/17156454-9808625
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1465-6914
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
7
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
R116
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
Generation of medaka gene knockout models by target-selected mutagenesis.
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