Linked Life Data

17135461

Source:http://linkedlifedata.com/resource/pubmed/id/17135461

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pubmed-article:17135461pubmed:abstractTextThe tuatara (Sphenodon punctatus) is of "extraordinary biological interest" as the most distinctive surviving reptilian lineage (Rhyncocephalia) in the world. To provide a genomic resource for an understanding of genome evolution in reptiles, and as part of a larger project to produce genomic resources for various reptiles (evogen.jgi.doe.gov/second_levels/BACs/our_libraries.html), a large-insert bacterial artificial chromosome (BAC) library from a male tuatara was constructed. The library consists of 215 424 individual clones whose average insert size was empirically determined to be 145 kb, yielding a genomic coverage of approximately 6.3x. A BAC-end sequencing analysis of 121 420 bp of sequence revealed a genomic GC content of 46.8%, among the highest observed thus far for vertebrates, and identified several short interspersed repetitive elements (mammalian interspersed repeat-type repeats) and long interspersed repetitive elements, including chicken repeat 1 element. Finally, as a quality control measure the arrayed library was screened with probes corresponding to 2 conserved noncoding regions of the candidate sex-determining gene DMRT1 and the DM domain of the related DMRT2 gene. A deep coverage contig spanning nearly 300 kb was generated, supporting the deep coverage and utility of the library for exploring tuatara genomics.lld:pubmed
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pubmed-article:17135461pubmed:authorpubmed-author:EdwardsScott...lld:pubmed
pubmed-article:17135461pubmed:authorpubmed-author:WangZhenshanZlld:pubmed
pubmed-article:17135461pubmed:authorpubmed-author:MiyakeTsutomu...lld:pubmed
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pubmed-article:17135461pubmed:dateRevised2007-12-3lld:pubmed
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pubmed-article:17135461pubmed:articleTitleTuatara (Sphenodon) genomics: BAC library construction, sequence survey, and application to the DMRT gene family.lld:pubmed
pubmed-article:17135461pubmed:affiliationDepartment of Biology, University of Washington, Seattle, WA 98195, USA. zhenshan@u.washington.edulld:pubmed
pubmed-article:17135461pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:17135461pubmed:publicationTypeResearch Support, U.S. Gov't, Non-P.H.S.lld:pubmed
pubmed-article:17135461pubmed:publicationTypeResearch Support, N.I.H., Extramurallld:pubmed
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