Linked Life Data

1712886

Source:http://linkedlifedata.com/resource/pubmed/id/1712886

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1991-8-16
pubmed:abstractText
Primary sensory neurons display various neuronal phenotypes which may be influenced by factors present in central or peripheral targets. In the case of DRG cells expressing substance P (SP), the influence of peripheral or central targets was tested on the neuronal expression of this neuropeptide. DRG cells were cultured from chick embryo at E6 or E10 (before or after establishment of functional connections with targets). Preprotachykinin mRNA was visualized in DRG cell cultures by either Northern blot or in situ hybridization using an antisense labeled riboprobe, while the neuropeptide SP was detected by immunostaining with a monoclonal antibody. In DRG cell cultures from E10, only 60% of neurons expressed SP. In contrast, DRG cell cultures performed at E6 showed a significant hybridization signal and SP-like immunoreactivity in virtually all the neurons (98%). The addition of extracts from muscle, skin, brain or spinal cord to DRG cells cultured at E6 reduced by 20% the percentage of neurons which express preprotachykinin mRNA and SP-like immunoreactivity. Our results indicate that factors issued from targets inhibit SP-expression by a subset of primary sensory neurons and act on the transcriptional control of preprotachykinin gene.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0169-328X
pubmed:author
pubmed:issnType
Print
pubmed:volume
10
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
107-14
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:1712886-Animals, pubmed-meshheading:1712886-Autoradiography, pubmed-meshheading:1712886-Blotting, Northern, pubmed-meshheading:1712886-Brain, pubmed-meshheading:1712886-Cells, Cultured, pubmed-meshheading:1712886-Chick Embryo, pubmed-meshheading:1712886-Chickens, pubmed-meshheading:1712886-DNA Replication, pubmed-meshheading:1712886-Ganglia, Spinal, pubmed-meshheading:1712886-Gene Expression, pubmed-meshheading:1712886-Gene Expression Regulation, pubmed-meshheading:1712886-Muscles, pubmed-meshheading:1712886-Neurons, Afferent, pubmed-meshheading:1712886-Phosphorus Radioisotopes, pubmed-meshheading:1712886-Protein Precursors, pubmed-meshheading:1712886-RNA, pubmed-meshheading:1712886-RNA, Messenger, pubmed-meshheading:1712886-RNA Probes, pubmed-meshheading:1712886-Skin Physiological Phenomena, pubmed-meshheading:1712886-Spinal Cord, pubmed-meshheading:1712886-Substance P, pubmed-meshheading:1712886-Sulfur Radioisotopes, pubmed-meshheading:1712886-Tachykinins, pubmed-meshheading:1712886-Tissue Extracts
pubmed:year
1991
pubmed:articleTitle
Expression of substance P and preprotachykinin mRNA by primary sensory neurons in culture: regulation by factors present in peripheral and central target tissues.
pubmed:affiliation
Institut d'Histologie et d'Embryologie, Faculté de Médicine, Université de Lausanne, Switzerland.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't