Linked Life Data

17116468

Source:http://linkedlifedata.com/resource/pubmed/id/17116468

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PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2006-11-22
pubmed:abstractText
Over the past decade, rapid, high-sensitivity mass spectrometric strat-egies have been developed and optimized for screening for the types of N- and O-glycans present in a diverse range of biological material, including secretions, cell lines, tissues, and organs. These glycomic strategies are based on matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass fingerprinting of permethylated derivatives, combined with electrospray (ES) or MALDI tandem mass spectrometry (MS/MS) sequencing and gas chromatography (GC)-MS linkage analysis, complemented by chemical and enzymatic degradations. Protocols for these methods are described in the first part of this chapter. Glycomic experiments yield large volumes of MS data, and interpretation of the resulting spectra remains a time-consuming bottleneck in the process. In the second part of this chapter, we describe the use and operation of a mass spectral viewer program capable of displaying and automatically labeling spectra arising from MALDI fingerprinting of N-glycans.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0076-6879
pubmed:author
pubmed:issnType
Print
pubmed:volume
415
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
59-86
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
Glycomic profiling of cells and tissues by mass spectrometry: fingerprinting and sequencing methodologies.
pubmed:affiliation
Department of Molecular Biosciences, Imperial College London, UK.
pubmed:publicationType
Journal Article