Linked Life Data

17114940

Source:http://linkedlifedata.com/resource/pubmed/id/17114940

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2007-8-20
pubmed:abstractText
In an earlier report two P-transposon insertion alleles of the noncoding hsromega gene, hsromega(05241) and P292 were shown to enhance neurodegeneration caused by expression of ataxin-1 protein with expanded poly-Q in a Drosophila model. In present study, we examined the possible relation between hsromega gene expression and toxicity due to poly-Q pathogenesis. The Drosophila hsromega gene produces several noncoding transcripts in almost all cell types, of which the >10 kb long hsromega-n transcript organizes heterogeneous RNA binding (hnRNPs) and related proteins as nucleoplasmic omega speckles. We show that P insertion alleles of the hsromega gene, which cause its overexpression, dominantly enhance neurodegeneration in fly eyes expressing either expanded poly-Q (127Q) or mutant huntingtin protein. Null allele of Hrb87F gene, encoding hnRNPA1, and a novel gene's mutant allele (l(3)pl10(R)), which affects the omega speckles, also dominantly enhance 127Q-induced neurodegeneration. The hsromega-n transcripts or the hnRNPs do not colocalize with the poly-Q nuclear inclusion bodies, neither in hsromega wild type, nor in hsromega mutant background. However, the levels of poly-Q and Hsp70 were significantly higher in hsromega mutant eye discs. Sequestration of hnRNPs and other related RNA-binding proteins by overexpression of hsromega transcripts in hsromega(05241) or in l(3)pl10(R) background or the reduced levels of Hrb87F protein seem to affect nuclear RNA metabolism, thus enhancing the toxicity due to poly-Q expansion.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1555-8584
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
3
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
28-35
pubmed:dateRevised
2010-1-15
pubmed:meshHeading
pubmed:articleTitle
Altered expressions of the noncoding hsromega gene enhances poly-Q-induced neurotoxicity in Drosophila.
pubmed:affiliation
Cytogenetics Laboratory, Department of Zoology, Banaras Hindu University, Varanasi, India.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't