Source:http://linkedlifedata.com/resource/pubmed/id/17114478
Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
11
|
pubmed:dateCreated |
2006-11-20
|
pubmed:abstractText |
According to the cancer immunoediting concept, inflammatory mediators play not only a critical role in promoting host protection against cancer but also contribute to cancer cell growth and survival. TNF-alpha is a critical factor in this network. However, the mechanisms underlying the tumor-promoting effect of TNF-alpha have not been fully elucidated yet. We previously reported that in vitro culture of Lewis lung carcinoma 3LL cells with TNF-alpha-producing macrophages resulted in enhanced resistance toward TNF-alpha-mediated lysis and increased malignancy of the 3LL cells. In this study, we analyzed the effects of endogenous TNF-alpha on TNF-alpha resistance and malignant behavior in vivo of low-malignant/TNF-alpha-sensitive 3LL-S cells and cancer cells derived from 3LL-S tumors that developed in wild-type or TNF-alpha(-/-) mice. Interestingly, 3LL-S cells acquired a malignant phenotype in vivo depending on the presence of host TNF-alpha, whereas acquisition of TNF-alpha resistance was TNF-alpha-independent. This result suggested that malignancy-promoting characteristics of 3LL-S cells other than TNF-alpha resistance are influenced in vivo by TNF-alpha. We previously identified the malignancy-promoting genes, secretory leukocyte protease inhibitor (SLPI) and S100A4, as being up-regulated in 3LL-S cells upon their s.c. growth in wild-type mice. In this study, we show that SLPI, but not S100A4, was induced in 3LL-S cells both in vitro and in vivo by TNF-alpha, and that silencing of in vivo induced 3LL-S SLPI expression using RNA interference abrogated in vivo progression but did not influence TNF-alpha resistance. These data indicate that SLPI induction may be one mechanism whereby TNF-alpha acts as an endogenous tumor promoter.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
AIM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering,
http://linkedlifedata.com/resource/pubmed/chemical/Secretory Leukocyte Peptidase...,
http://linkedlifedata.com/resource/pubmed/chemical/Slpi protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
|
pubmed:status |
MEDLINE
|
pubmed:month |
Dec
|
pubmed:issn |
0022-1767
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
1
|
pubmed:volume |
177
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
8046-52
|
pubmed:meshHeading |
pubmed-meshheading:17114478-Animals,
pubmed-meshheading:17114478-Carcinoma, Lewis Lung,
pubmed-meshheading:17114478-Cytotoxicity, Immunologic,
pubmed-meshheading:17114478-Female,
pubmed-meshheading:17114478-Gene Expression,
pubmed-meshheading:17114478-Mice,
pubmed-meshheading:17114478-Neoplasm Invasiveness,
pubmed-meshheading:17114478-RNA, Small Interfering,
pubmed-meshheading:17114478-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:17114478-Secretory Leukocyte Peptidase Inhibitor,
pubmed-meshheading:17114478-Tumor Necrosis Factor-alpha
|
pubmed:year |
2006
|
pubmed:articleTitle |
The tumor-promoting effect of TNF-alpha involves the induction of secretory leukocyte protease inhibitor.
|
pubmed:affiliation |
Department of Molecular and Cellular Interactions, Vlaams Interuniversitair Instituut voor Biotechnologie, Vrije Universiteit Brussel, Gebouw E, niveau 8, Pleinlaan 2, 1050 Brussels, Belgium. ndevoogd@vub.ac.be
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|