Linked Life Data

17113874

Source:http://linkedlifedata.com/resource/pubmed/id/17113874

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2006-11-20
pubmed:abstractText
The identification of cDNAs encoding glycosyltransferases and carbohydrate-modifying enzymes such as sulfotransferases has allowed expression of a given enzyme in cells that lack the enzyme or express it at very low levels. By comparing the function and/or structure of carbohydrates expressed in cells before and after transfection, we can determine the function of the ectopically expressed enzyme. This assay is less time consuming than assaying function by obtaining cells deficient in a given enzyme. Moreover, it is a more definitive method for establishing the function of the enzyme because the result is derived from an enzyme introduced by transfection. Using this method, an enormous amount of knowledge relevant to the structure and function of glycoenzymes has been derived from such studies. In this chapter, we describe methods used to obtain mammalian cells that have acquired new carbohydrate structures and function following transfection of mammalian expression vectors harboring glycoenzymes.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0076-6879
pubmed:author
pubmed:issnType
Print
pubmed:volume
416
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
293-304
pubmed:dateRevised
2007-12-3
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
Expression of specific carbohydrates by transfection with carbohydrate modifying enzymes.
pubmed:affiliation
Division of Glyco-Signal Research, Institute of Molecular Biomembrane and Glycobiology, Tohoku Pharmaceutical University, Sendai, Japan.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural