Linked Life Data

17108334

Source:http://linkedlifedata.com/resource/pubmed/id/17108334

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2007-2-21
pubmed:abstractText
Macrophages are a major component of the leukocyte population of human pregnant endometrium. Although several crucial functions have been ascribed to these cells, the mechanisms underlying macrophage trafficking in the placental bed are poorly understood. The aim of this study was to evaluate the in vivo expression of two potentially antagonistic macrophage-targeting chemokines, colony stimulating factor 1 (CSF1, also known as M-CSF) and macrophage migration inhibitory factor (MIF), in term decidua, and to examine the effects of the inflammatory cytokines tumor necrosis factor (TNF, also known as TNF alpha) and interleukin 1beta (IL1B) on CSF1 and MIF expression in cultured decidual cells. The expression of CSF1 and MIF in term decidua was evaluated by immunohistochemistry. Cultured decidual cells were primed with estradiol (E2) or with E2+medroxyprogesterone acetate (MPA), and then incubated with corresponding steroid(s) with or without TNF or IL1B. The levels of CSF1 and MIF protein and mRNA were assessed by ELISA and quantitative RT-PCR, respectively. Immunostaining for CSF1 and MIF was observed in term decidua. The levels of secreted CSF1 and MIF were similarly unchanged whether the decidual cells were incubated with E2 or with E2+MPA. The CSF1 levels significantly increased in cultures exposed to E2 or E2+MPA plus TNF or IL1B. In contrast, the MIF levels in TNF- and IL1B-treated cells were not changed significantly from the control cultures. The ELISA data were confirmed by quantitative RT-PCR analysis. These results indicate that CSF1 and MIF are involved in regulating macrophage trafficking at the fetal-maternal interface, and suggest a mechanism by which inflammatory cytokines influence pregnancy by regulating decidual macrophage infiltration.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0006-3363
pubmed:author
pubmed:issnType
Print
pubmed:volume
76
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
433-9
pubmed:dateRevised
2007-12-3
pubmed:meshHeading
pubmed-meshheading:17108334-Cell Movement, pubmed-meshheading:17108334-Cells, Cultured, pubmed-meshheading:17108334-Cytokines, pubmed-meshheading:17108334-Decidua, pubmed-meshheading:17108334-Female, pubmed-meshheading:17108334-Gene Expression Regulation, Developmental, pubmed-meshheading:17108334-Humans, pubmed-meshheading:17108334-Inflammation Mediators, pubmed-meshheading:17108334-Interleukin-1beta, pubmed-meshheading:17108334-Macrophage Colony-Stimulating Factor, pubmed-meshheading:17108334-Macrophage Migration-Inhibitory Factors, pubmed-meshheading:17108334-Macrophages, pubmed-meshheading:17108334-Placental Circulation, pubmed-meshheading:17108334-Pregnancy, pubmed-meshheading:17108334-Pregnancy Trimester, Third, pubmed-meshheading:17108334-RNA, Messenger, pubmed-meshheading:17108334-Tumor Necrosis Factor-alpha
pubmed:year
2007
pubmed:articleTitle
Differential regulation of colony stimulating factor 1 and macrophage migration inhibitory factor expression by inflammatory cytokines in term human decidua: implications for macrophage trafficking at the fetal-maternal interface.
pubmed:affiliation
Department of Human Pathology and Oncology, University of Siena, 53100 Siena, Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural