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rdf:type |
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lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0029282,
umls-concept:C0031715,
umls-concept:C0109317,
umls-concept:C0172537,
umls-concept:C0185117,
umls-concept:C0752312,
umls-concept:C1150579,
umls-concept:C1333340,
umls-concept:C1366876,
umls-concept:C1366882,
umls-concept:C1370600,
umls-concept:C1514559,
umls-concept:C1705767,
umls-concept:C1705791,
umls-concept:C1833235,
umls-concept:C2349975,
umls-concept:C2911684
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pubmed:issue |
2
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pubmed:dateCreated |
2007-2-6
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pubmed:abstractText |
In the present study, we investigated the relationship between ornithine decarboxylase, MAP kinase, and MMP-2 expression in vitro. Overexpression of ornithine decarboxylase cDNA induced MMP-2 expression both at mRNA and protein levels. Promoter analysis and gel shift assay showed that p53 and Ets-1 were involved in MMP-2 expression in ornithine decarboxylase overexpressing transfectants. Erk and p38 MAP kinase were significantly activated. Using specific inhibitors of MEK and p38, we clarified that MMP-2 expression was induced via both Erk and p38 MAP kinase signaling pathways. This is the first report showing the existence of a causal relationship between ornithine decarboxylase expression, Erk and p38 MAP kinase activation, and MMP-2 expression.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1065-6995
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
31
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
141-7
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:17088079-Animals,
pubmed-meshheading:17088079-COS Cells,
pubmed-meshheading:17088079-Cercopithecus aethiops,
pubmed-meshheading:17088079-Extracellular Signal-Regulated MAP Kinases,
pubmed-meshheading:17088079-Gene Expression,
pubmed-meshheading:17088079-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:17088079-Humans,
pubmed-meshheading:17088079-Matrix Metalloproteinase 2,
pubmed-meshheading:17088079-Ornithine Decarboxylase,
pubmed-meshheading:17088079-Phosphorylation,
pubmed-meshheading:17088079-Promoter Regions, Genetic,
pubmed-meshheading:17088079-Protein Kinase Inhibitors,
pubmed-meshheading:17088079-RNA, Messenger,
pubmed-meshheading:17088079-p38 Mitogen-Activated Protein Kinases
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pubmed:year |
2007
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pubmed:articleTitle |
Ornithine decarboxylase overexpression enhances ERK and p38 phosphorylation and matrix metalloproteinase-2 expression.
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pubmed:affiliation |
Department of Physiological Chemistry and Metabolism, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Tokyo 113-0033, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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