|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
1991-5-28
|
|
pubmed:abstractText |
A specific enzyme-linked immunoassay (ELISA) has been developed for the determination of neutrophil proteinase 4 (NP4) in human plasma/serum and tissue fluids. Comparison of the sequence for the first 20 N-terminal amino acids of NP4, neutrophil elastase and cathepsin G shows that NP4 is distinct from the other two proteases. However, all three show considerable homology. Neither elastase nor cathepsin G show any immunoreactivity when tested in the present ELISA. Normal human plasma contains about 38 micrograms/l of NP4, identified as alpha 1-proteinase inhibitor complexes. This represents about 50% of the total amount of NP4 released in plasma. The remaining 50% is bound by alpha 2-macroglobulin. Blood coagulation leads to a rapid release of NP4 from the leukocytes. Peritonitis is accompanied by a pronounced release of NP4, as shown by a three-to 10-fold increase of NP4 plasma levels and by the NP4 level in peritoneal exudates, which reaches about 40 mg/l in severe cases.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Feb
|
|
pubmed:issn |
0036-5513
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
51
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
23-9
|
|
pubmed:dateRevised |
2009-11-19
|
|
pubmed:meshHeading |
pubmed-meshheading:1708522-Amino Acid Sequence,
pubmed-meshheading:1708522-Cathepsin G,
pubmed-meshheading:1708522-Cathepsins,
pubmed-meshheading:1708522-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:1708522-Female,
pubmed-meshheading:1708522-Humans,
pubmed-meshheading:1708522-Leukocyte Elastase,
pubmed-meshheading:1708522-Male,
pubmed-meshheading:1708522-Molecular Sequence Data,
pubmed-meshheading:1708522-Myeloblastin,
pubmed-meshheading:1708522-Neutrophils,
pubmed-meshheading:1708522-Pancreatic Elastase,
pubmed-meshheading:1708522-Peritonitis,
pubmed-meshheading:1708522-Reference Values,
pubmed-meshheading:1708522-Sequence Homology, Nucleic Acid,
pubmed-meshheading:1708522-Serine Endopeptidases,
pubmed-meshheading:1708522-alpha 1-Antitrypsin,
pubmed-meshheading:1708522-alpha-Macroglobulins
|
|
pubmed:year |
1991
|
|
pubmed:articleTitle |
Release of immunoreactive human neutrophil proteinase 4, normally and in peritonitis.
|
|
pubmed:affiliation |
University of Lund, Department of Surgical Pathophysiology, Malmö General Hospital, Sweden.
|
|
pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|
