Linked Life Data

1704199

Source:http://linkedlifedata.com/resource/pubmed/id/1704199

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1991-3-8
pubmed:abstractText
A simple definition for pause strength (tau i) has been given, quantitatively describing transcriptional pausing of RNA polymerases. It permits derivation of practical assessments, based on single-round transcription reactions, which measure the average time a polymerase stops in vitro at certain sites during transcription elongation. We demonstrate that pause strengths can be determined with high accuracy when transcription elongation is started simultaneously from radioactively labeled and purified ternary complexes and transcripts are separated on sequencing gels. Our concept is exemplified by measuring pause strengths on supercoiled templates in the leader region of the Escherichia coli rrnB operon in the presence and the absence of the transcription factor NusA.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0003-2697
pubmed:author
pubmed:issnType
Print
pubmed:volume
189
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
254-61
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
A quantitative assessment for transcriptional pausing of DNA-dependent RNA polymerases in vitro.
pubmed:affiliation
Institut für Physikalische Biologie, Heinrich-Heine-Universität Düsseldorf, Federal Republic of Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't