Linked Life Data

17028301

Source:http://linkedlifedata.com/resource/pubmed/id/17028301

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2007-1-8
pubmed:abstractText
Numerous studies have shown that drugs of abuse induce changes in protein expression in the brain that are thought to play a role in synaptic plasticity. Drug-induced plasticity can be mediated by changes at the synapse and more specifically at the postsynaptic density (PSD), which receives and transduces synaptic information. To date, the majority of studies examining synaptic protein profiles have focused on identifying the synaptic proteome. Only a handful of studies have examined the changes in synaptic profile by drug administration. We applied a quantitative proteomics analysis technique with the cleavable ICAT reagent to quantitate relative changes in protein levels of the hippocampal PSD in response to morphine administration. We identified a total of 102 proteins in the mouse hippocampal PSD. The majority of these were signaling, trafficking, and cytoskeletal proteins involved in synaptic plasticity, learning, and memory. Among the proteins whose levels were found to be altered by morphine administration, clathrin levels were increased to the largest extent. Immunoblotting and electron microscopy studies showed that this increase was localized to the PSD. Morphine treatment was also found to lead to a local increase in two other components of the endocytic machinery, dynamin and AP-2, suggesting a critical involvement of the endocytic machinery in the modulatory effects of morphine. Because alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors are thought to undergo clathrin-mediated endocytosis, we examined the effect of morphine administration on the association of the AMPA receptor subunit, GluR1, with clathrin. We found a substantial decrease in the levels of GluR1 associated with clathrin. Taken together, these results suggest that, by causing a redistribution of endocytic proteins at the synapse, morphine modulates synaptic plasticity at hippocampal glutamatergic synapses.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1535-9476
pubmed:author
pubmed:issnType
Print
pubmed:volume
6
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
29-42
pubmed:dateRevised
2007-12-3
pubmed:meshHeading
pubmed-meshheading:17028301-Amino Acid Sequence, pubmed-meshheading:17028301-Animals, pubmed-meshheading:17028301-Blotting, Western, pubmed-meshheading:17028301-Chromatography, Liquid, pubmed-meshheading:17028301-Clathrin Heavy Chains, pubmed-meshheading:17028301-Endocytosis, pubmed-meshheading:17028301-Hippocampus, pubmed-meshheading:17028301-Mass Spectrometry, pubmed-meshheading:17028301-Mice, pubmed-meshheading:17028301-Mice, Inbred C57BL, pubmed-meshheading:17028301-Molecular Sequence Data, pubmed-meshheading:17028301-Morphine, pubmed-meshheading:17028301-Nerve Tissue Proteins, pubmed-meshheading:17028301-Protein Binding, pubmed-meshheading:17028301-Proteomics, pubmed-meshheading:17028301-Receptors, AMPA, pubmed-meshheading:17028301-Reproducibility of Results, pubmed-meshheading:17028301-Vesicular Transport Proteins
pubmed:year
2007
pubmed:articleTitle
Morphine administration alters the profile of hippocampal postsynaptic density-associated proteins: a proteomics study focusing on endocytic proteins.
pubmed:affiliation
Department of Pharmacology and Biological Chemistry, Mount Sinai School of Medicine, New York, New York 10029, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, N.I.H., Extramural