Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 10
pubmed:dateCreated
2006-9-26
pubmed:abstractText
This paper describes the developments, role and contributions of the NMR spectroscopy groups in the Structural Proteomics In Europe (SPINE) consortium. Focusing on the development of high-throughput (HTP) pipelines for NMR structure determinations of proteins, all aspects from sample preparation, data acquisition, data processing, data analysis to structure determination have been improved with respect to sensitivity, automation, speed, robustness and validation. Specific highlights are protonless (13)C-direct detection methods and inferential structure determinations (ISD). In addition to technological improvements, these methods have been applied to deliver over 60 NMR structures of proteins, among which are five that failed to crystallize. The inclusion of NMR spectroscopy in structural proteomics pipelines improves the success rate for protein structure determinations.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0907-4449
pubmed:author
pubmed-author:AcFF, pubmed-author:AtkinsonA RAR, pubmed-author:BanciLL, pubmed-author:BertiniII, pubmed-author:BrunnerKK, pubmed-author:Ciofi-BaffoniSS, pubmed-author:DötschVV, pubmed-author:DiercksTT, pubmed-author:EngelkeFF, pubmed-author:FolkersG EGE, pubmed-author:GüntherUU, pubmed-author:GriesingerCC, pubmed-author:GronwaldWW, pubmed-author:HabeckMM, pubmed-author:KalbitzerH RHR, pubmed-author:KapteinRR, pubmed-author:KiefferBB, pubmed-author:LeeflangB RBR, pubmed-author:LossSS, pubmed-author:LuchinatCC, pubmed-author:MarquardsenTT, pubmed-author:MoskauDD, pubmed-author:NeidigK PKP, pubmed-author:NilgesMM, pubmed-author:PiccioliMM, pubmed-author:PierattelliRR, pubmed-author:RiepingWW, pubmed-author:SchippmannTT, pubmed-author:SchwalbeHH, pubmed-author:TravéGG, pubmed-author:TrennerJJ, pubmed-author:WöhnertJJ, pubmed-author:ZweckstetterMM, pubmed-author:de JongR NRN
pubmed:issnType
Print
pubmed:volume
62
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1150-61
pubmed:dateRevised
2007-7-24
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
NMR in the SPINE Structural Proteomics project.
pubmed:affiliation
Bijvoet Center for Biomolecular Research, NMR Spectroscopy, Utrecht University, Padualaan 8, CH Utrecht, The Netherlands.
pubmed:publicationType
Journal Article, Review, Research Support, Non-U.S. Gov't