| pubmed-article:16952378 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16952378 | lifeskim:mentions | umls-concept:C0036536 | lld:lifeskim |
| pubmed-article:16952378 | lifeskim:mentions | umls-concept:C0021760 | lld:lifeskim |
| pubmed-article:16952378 | lifeskim:mentions | umls-concept:C0206131 | lld:lifeskim |
| pubmed-article:16952378 | lifeskim:mentions | umls-concept:C0036537 | lld:lifeskim |
| pubmed-article:16952378 | lifeskim:mentions | umls-concept:C0389071 | lld:lifeskim |
| pubmed-article:16952378 | lifeskim:mentions | umls-concept:C0001047 | lld:lifeskim |
| pubmed-article:16952378 | lifeskim:mentions | umls-concept:C0030190 | lld:lifeskim |
| pubmed-article:16952378 | lifeskim:mentions | umls-concept:C1456820 | lld:lifeskim |
| pubmed-article:16952378 | lifeskim:mentions | umls-concept:C0392747 | lld:lifeskim |
| pubmed-article:16952378 | lifeskim:mentions | umls-concept:C0599946 | lld:lifeskim |
| pubmed-article:16952378 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:16952378 | lifeskim:mentions | umls-concept:C0443172 | lld:lifeskim |
| pubmed-article:16952378 | pubmed:issue | 25 | lld:pubmed |
| pubmed-article:16952378 | pubmed:dateCreated | 2006-11-14 | lld:pubmed |
| pubmed-article:16952378 | pubmed:abstractText | TNF-alpha is a key molecule in obesity-related metabolic disturbances. This study was designed to determine whether N-acetylcysteine (NAC), an antioxidant, prevents the activation of nuclear factor-kappaB (NF-kappaB) by exogenously administered TNF-alpha in adipocytes, and whether such change affects the production of adipocytokines. The treatment of well-differentiated 3T3-L1 cells with 20 mM of NAC significantly increased the reduced glutathione concentration up to 150% of control. The treatment with 10 ng/ml of TNF-alpha decreased antioxidant enzyme levels such as CuZn-superoxide dismutase (SOD), MnSOD and catalase, and activated NF-kappaB in 3T3-L1 adipocytes. The activation of NF-kappaB was significantly prevented by the pretreatment with 20 mM of NAC. TNF-alpha (1-10 ng/ml) dose-dependently increased interleukin (IL)-6 and plasminogen activator inhibitor-1 (PAI-1) secretion from 3T3-L1 adipocytes, while decreased adiponectin secretion. NAC (5-20 mM) attenuated the TNF-alpha-induced changes in these adipocytokine secretions in a dose-dependent manner. The effect of TNF-alpha and NAC on the adipocytokine productions was exerted at the m-RNA level, judging from results of the real time RT-PCR analysis. The present study revealed that NAC inhibited the TNF-alpha-mediated activation of NF-kappaB and improved the adverse changes in the levels of IL-6, PAI-1 and adiponectin in 3T3-L1 adipocytes. NAC may have the potential to improve the obesity-related abnormal adipocytokine metabolism by attenuating the TNF-alpha-induced oxidant-antioxidant imbalance in adipocytes. | lld:pubmed |
| pubmed-article:16952378 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16952378 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16952378 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16952378 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16952378 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16952378 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16952378 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16952378 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16952378 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16952378 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16952378 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16952378 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16952378 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16952378 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16952378 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16952378 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:16952378 | pubmed:issn | 0024-3205 | lld:pubmed |
| pubmed-article:16952378 | pubmed:author | pubmed-author:YamamotoYukiy... | lld:pubmed |
| pubmed-article:16952378 | pubmed:author | pubmed-author:AsayamaKohtar... | lld:pubmed |
| pubmed-article:16952378 | pubmed:author | pubmed-author:ShirahataAkir... | lld:pubmed |
| pubmed-article:16952378 | pubmed:author | pubmed-author:DobashiKazush... | lld:pubmed |
| pubmed-article:16952378 | pubmed:author | pubmed-author:ArakiShunsuke... | lld:pubmed |
| pubmed-article:16952378 | pubmed:author | pubmed-author:KuboKazuyasuK | lld:pubmed |
| pubmed-article:16952378 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16952378 | pubmed:day | 17 | lld:pubmed |
| pubmed-article:16952378 | pubmed:volume | 79 | lld:pubmed |
| pubmed-article:16952378 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16952378 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16952378 | pubmed:pagination | 2405-12 | lld:pubmed |
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| pubmed-article:16952378 | pubmed:meshHeading | pubmed-meshheading:16952378... | lld:pubmed |
| pubmed-article:16952378 | pubmed:meshHeading | pubmed-meshheading:16952378... | lld:pubmed |
| pubmed-article:16952378 | pubmed:meshHeading | pubmed-meshheading:16952378... | lld:pubmed |
| pubmed-article:16952378 | pubmed:meshHeading | pubmed-meshheading:16952378... | lld:pubmed |
| pubmed-article:16952378 | pubmed:meshHeading | pubmed-meshheading:16952378... | lld:pubmed |
| pubmed-article:16952378 | pubmed:meshHeading | pubmed-meshheading:16952378... | lld:pubmed |
| pubmed-article:16952378 | pubmed:meshHeading | pubmed-meshheading:16952378... | lld:pubmed |
| pubmed-article:16952378 | pubmed:meshHeading | pubmed-meshheading:16952378... | lld:pubmed |
| pubmed-article:16952378 | pubmed:year | 2006 | lld:pubmed |
| pubmed-article:16952378 | pubmed:articleTitle | N-acetylcysteine attenuates TNF-alpha induced changes in secretion of interleukin-6, plasminogen activator inhibitor-1 and adiponectin from 3T3-L1 adipocytes. | lld:pubmed |
| pubmed-article:16952378 | pubmed:affiliation | Department of Pediatrics, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu, 807-8555, Japan. | lld:pubmed |
| pubmed-article:16952378 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16952378 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16952378 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16952378 | lld:pubmed |
