Linked Life Data

1692513

Source:http://linkedlifedata.com/resource/pubmed/id/1692513

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1990-6-20
pubmed:abstractText
This study demonstrates that endocytosis in the oocyte of Drosophila melanogaster is reversibly blocked at the stage of pit formation by the temperature-sensitive, single-gene mutant, shibirts1. Uptake of horeradish peroxidase conjugated with wheat-germ agglutinin was observed to be normal in mutant oocytes at 19 degrees C, but was blocked at 29 degrees C. After 10 min at 29 degrees C, there was a build-up of coated pits along invaginations of the plasma membrane. Also, the endosomal compartment consisting of tubules, bulbs, and small yolk spheres, disappeared. Lowering the temperature to 19 degrees C after 10 min at 29 degrees C released a synchronized wave of endocytosis into a cytoplasm cleared of uptake-related organelles. By observing this synchronized wave after exposure to 19 degrees C for varying durations, we determined that endocytosis proceeds as follows: coated pits/vesicles----tubules----small yolk spheres----mature yolk spheres. The observations suggest that these organelles transform one into another within this sequence.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0302-766X
pubmed:author
pubmed:issnType
Print
pubmed:volume
259
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
199-207
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Synchronized endocytosis studied in the oocyte of a temperature-sensitive mutant of Drosophila melanogaster.
pubmed:affiliation
Division of Neurosciences, Beckman Research Institute of the City of Hope, Duarte, California 91010.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.