| pubmed-article:1690717 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1690717 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:1690717 | lifeskim:mentions | umls-concept:C0079459 | lld:lifeskim |
| pubmed-article:1690717 | lifeskim:mentions | umls-concept:C1518174 | lld:lifeskim |
| pubmed-article:1690717 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:1690717 | lifeskim:mentions | umls-concept:C1333104 | lld:lifeskim |
| pubmed-article:1690717 | lifeskim:mentions | umls-concept:C0086860 | lld:lifeskim |
| pubmed-article:1690717 | lifeskim:mentions | umls-concept:C0439064 | lld:lifeskim |
| pubmed-article:1690717 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:1690717 | lifeskim:mentions | umls-concept:C0013879 | lld:lifeskim |
| pubmed-article:1690717 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:1690717 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:1690717 | pubmed:issue | 10 | lld:pubmed |
| pubmed-article:1690717 | pubmed:dateCreated | 1990-5-3 | lld:pubmed |
| pubmed-article:1690717 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1690717 | pubmed:abstractText | Some human carcinoma cells constitutively produce granulocyte colony-stimulating factor (G-CSF) which stimulates the proliferation and differentiation of the progenitor cells of neutrophilic granulocytes. By introducing mouse G-CSF chromosomal gene or its promoter DNA into human carcinoma cell lines of CHU-2, SK-HEP-1, and U-87MG, it was shown that the constitutive expression of G-CSF in these carcinoma cells was due to the intrinsic activation of nuclear factors which work on the promoter region of the G-CSF gene. A series of 5' deletion mutants, linker scanning mutants, and internal deletion mutants was constructed in the promoter of mouse G-CSF gene and was introduced into human CHU-2 cells to analyze their promoter activities. These studies demonstrated that at least three regulatory elements in the promoter of the G-CSF gene are essential for the constitutive expression of G-CSF in CHU-2 cells. These elements include the consensus decanucleotide "GAGRTTCCA/CC" present on G-CSF, granulocyte/macrophage colony-stimulating factor, and interleukin 3 genes and the "ATTTGCAT" octamer transcription factor binding site. Some point mutations in these consensus sequences significantly diminished the promoter activity in CHU-2 cells. | lld:pubmed |
| pubmed-article:1690717 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1690717 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1690717 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1690717 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1690717 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1690717 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1690717 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1690717 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1690717 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1690717 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:1690717 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:1690717 | pubmed:author | pubmed-author:TsuchiyaMM | lld:pubmed |
| pubmed-article:1690717 | pubmed:author | pubmed-author:NagataSS | lld:pubmed |
| pubmed-article:1690717 | pubmed:author | pubmed-author:NishizawaMM | lld:pubmed |
| pubmed-article:1690717 | pubmed:author | pubmed-author:Watanabe-Fuku... | lld:pubmed |
| pubmed-article:1690717 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1690717 | pubmed:day | 5 | lld:pubmed |
| pubmed-article:1690717 | pubmed:volume | 265 | lld:pubmed |
| pubmed-article:1690717 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1690717 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1690717 | pubmed:pagination | 5897-902 | lld:pubmed |
| pubmed-article:1690717 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
| pubmed-article:1690717 | pubmed:meshHeading | pubmed-meshheading:1690717-... | lld:pubmed |
| pubmed-article:1690717 | pubmed:meshHeading | pubmed-meshheading:1690717-... | lld:pubmed |
| pubmed-article:1690717 | pubmed:meshHeading | pubmed-meshheading:1690717-... | lld:pubmed |
| pubmed-article:1690717 | pubmed:meshHeading | pubmed-meshheading:1690717-... | lld:pubmed |
| pubmed-article:1690717 | pubmed:meshHeading | pubmed-meshheading:1690717-... | lld:pubmed |
| pubmed-article:1690717 | pubmed:meshHeading | pubmed-meshheading:1690717-... | lld:pubmed |
| pubmed-article:1690717 | pubmed:meshHeading | pubmed-meshheading:1690717-... | lld:pubmed |
| pubmed-article:1690717 | pubmed:meshHeading | pubmed-meshheading:1690717-... | lld:pubmed |
| pubmed-article:1690717 | pubmed:meshHeading | pubmed-meshheading:1690717-... | lld:pubmed |
| pubmed-article:1690717 | pubmed:meshHeading | pubmed-meshheading:1690717-... | lld:pubmed |
| pubmed-article:1690717 | pubmed:meshHeading | pubmed-meshheading:1690717-... | lld:pubmed |
| pubmed-article:1690717 | pubmed:meshHeading | pubmed-meshheading:1690717-... | lld:pubmed |
| pubmed-article:1690717 | pubmed:meshHeading | pubmed-meshheading:1690717-... | lld:pubmed |
| pubmed-article:1690717 | pubmed:meshHeading | pubmed-meshheading:1690717-... | lld:pubmed |
| pubmed-article:1690717 | pubmed:meshHeading | pubmed-meshheading:1690717-... | lld:pubmed |
| pubmed-article:1690717 | pubmed:meshHeading | pubmed-meshheading:1690717-... | lld:pubmed |
| pubmed-article:1690717 | pubmed:meshHeading | pubmed-meshheading:1690717-... | lld:pubmed |
| pubmed-article:1690717 | pubmed:meshHeading | pubmed-meshheading:1690717-... | lld:pubmed |
| pubmed-article:1690717 | pubmed:meshHeading | pubmed-meshheading:1690717-... | lld:pubmed |
| pubmed-article:1690717 | pubmed:meshHeading | pubmed-meshheading:1690717-... | lld:pubmed |
| pubmed-article:1690717 | pubmed:meshHeading | pubmed-meshheading:1690717-... | lld:pubmed |
| pubmed-article:1690717 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:1690717 | pubmed:articleTitle | Multiple elements in the promoter of granulocyte colony-stimulating factor gene regulate its constitutive expression in human carcinoma cells. | lld:pubmed |
| pubmed-article:1690717 | pubmed:affiliation | Osaka Bioscience Institute, Japan. | lld:pubmed |
| pubmed-article:1690717 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1690717 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1690717 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1690717 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1690717 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1690717 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1690717 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1690717 | lld:pubmed |
