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rdf:type |
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lifeskim:mentions |
umls-concept:C0013879,
umls-concept:C0017262,
umls-concept:C0079459,
umls-concept:C0086418,
umls-concept:C0086860,
umls-concept:C0185117,
umls-concept:C0439064,
umls-concept:C0851285,
umls-concept:C1333104,
umls-concept:C1518174,
umls-concept:C2911684
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pubmed:issue |
10
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pubmed:dateCreated |
1990-5-3
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pubmed:databankReference |
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pubmed:abstractText |
Some human carcinoma cells constitutively produce granulocyte colony-stimulating factor (G-CSF) which stimulates the proliferation and differentiation of the progenitor cells of neutrophilic granulocytes. By introducing mouse G-CSF chromosomal gene or its promoter DNA into human carcinoma cell lines of CHU-2, SK-HEP-1, and U-87MG, it was shown that the constitutive expression of G-CSF in these carcinoma cells was due to the intrinsic activation of nuclear factors which work on the promoter region of the G-CSF gene. A series of 5' deletion mutants, linker scanning mutants, and internal deletion mutants was constructed in the promoter of mouse G-CSF gene and was introduced into human CHU-2 cells to analyze their promoter activities. These studies demonstrated that at least three regulatory elements in the promoter of the G-CSF gene are essential for the constitutive expression of G-CSF in CHU-2 cells. These elements include the consensus decanucleotide "GAGRTTCCA/CC" present on G-CSF, granulocyte/macrophage colony-stimulating factor, and interleukin 3 genes and the "ATTTGCAT" octamer transcription factor binding site. Some point mutations in these consensus sequences significantly diminished the promoter activity in CHU-2 cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
5
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pubmed:volume |
265
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5897-902
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:1690717-Adenocarcinoma,
pubmed-meshheading:1690717-Animals,
pubmed-meshheading:1690717-Base Sequence,
pubmed-meshheading:1690717-Carcinoma, Squamous Cell,
pubmed-meshheading:1690717-Colony-Stimulating Factors,
pubmed-meshheading:1690717-Deoxyribonuclease BamHI,
pubmed-meshheading:1690717-Deoxyribonucleases, Type II Site-Specific,
pubmed-meshheading:1690717-Gene Expression Regulation,
pubmed-meshheading:1690717-Glioma,
pubmed-meshheading:1690717-Granulocyte Colony-Stimulating Factor,
pubmed-meshheading:1690717-Humans,
pubmed-meshheading:1690717-Mice,
pubmed-meshheading:1690717-Molecular Sequence Data,
pubmed-meshheading:1690717-Mutation,
pubmed-meshheading:1690717-Neoplasms,
pubmed-meshheading:1690717-Plasmids,
pubmed-meshheading:1690717-Promoter Regions, Genetic,
pubmed-meshheading:1690717-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:1690717-Restriction Mapping,
pubmed-meshheading:1690717-Transfection,
pubmed-meshheading:1690717-Tumor Cells, Cultured
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pubmed:year |
1990
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pubmed:articleTitle |
Multiple elements in the promoter of granulocyte colony-stimulating factor gene regulate its constitutive expression in human carcinoma cells.
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pubmed:affiliation |
Osaka Bioscience Institute, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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