Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2006-10-18
pubmed:abstractText
The Golgi microtubule-associated protein of 210kDa (GMAP-210) has been shown to play a role in the assembly and maintenance of the Golgi apparatus in mammalian tissue culture cells. To develop a genetic model to study the function of GMAP-210 in vivo, we identified its closest relative in Drosophila melanogaster, dGMAP. We show that the dGMAP gene encodes two alternatively spliced transcripts, only one of which is translated into a protein product. To gain insight into the role of dGMAP, we generated a polyclonal antibody and investigated the protein distribution during development. This gene is ubiquitously expressed during embryonic and larval development with the highest level in polar cells, gut and salivary glands. We further show that dGMAP is present in the Golgi apparatus, and using electron microscopy of salivary glands, we observed a preferential localization at the cis side and at the rims of the Golgi stacks. Finally, we demonstrate that overexpression of dGMAP in salivary glands impairs Golgi architecture and function, whereas RNAi-mediated depletion of dGMAP does not induce any structural modification of the Golgi apparatus, and anterograde transport is seemingly unaffected. Altogether our results suggest that dGMAP is the ortholog of mammalian GMAP-210.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0171-9335
pubmed:author
pubmed:issnType
Print
pubmed:volume
85
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1155-66
pubmed:meshHeading
pubmed-meshheading:16904228-Alternative Splicing, pubmed-meshheading:16904228-Amino Acid Sequence, pubmed-meshheading:16904228-Animals, pubmed-meshheading:16904228-Base Sequence, pubmed-meshheading:16904228-Biological Transport, pubmed-meshheading:16904228-Cells, Cultured, pubmed-meshheading:16904228-Drosophila, pubmed-meshheading:16904228-Drosophila Proteins, pubmed-meshheading:16904228-Embryo, Nonmammalian, pubmed-meshheading:16904228-Embryonic Development, pubmed-meshheading:16904228-Fluorescent Antibody Technique, pubmed-meshheading:16904228-Golgi Apparatus, pubmed-meshheading:16904228-Humans, pubmed-meshheading:16904228-Microtubule-Associated Proteins, pubmed-meshheading:16904228-Molecular Sequence Data, pubmed-meshheading:16904228-Nuclear Proteins, pubmed-meshheading:16904228-Protein Biosynthesis, pubmed-meshheading:16904228-Protein Isoforms
pubmed:year
2006
pubmed:articleTitle
The cis-Golgi Drosophila GMAP has a role in anterograde transport and Golgi organization in vivo, similar to its mammalian ortholog in tissue culture cells.
pubmed:affiliation
Institut de Recherches Signalisation, Biologie du Développement et Cancer, CNRS UMR 6543, Centre de Biochimie, Parc Valrose, F-06108 Nice Cedex 2, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't