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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1990-2-23
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pubmed:abstractText |
We report the functional expression of the mammalian muscle-adipocyte insulin-sensitive hexose transporter in Xenopus laevis oocytes. Oocytes microinjected with RNA synthesized in vitro showed enhanced hexose transport activity compared with uninjected controls. However, like the endogenous oocyte hexose transporter, activity was stimulated only twofold by 1 microM insulin. X. laevis oocytes injected with in vitro-synthesized RNA encoding the human insulin proreceptor expressed a functionally active insulin receptor that enhanced the insulin sensitivity of injected oocytes. This increase was not observed in oocytes expressing a mutant insulin receptor that lacked protein tyrosine kinase activity. In the presence of the coexpressed human insulin receptor, insulin induced a two- to threefold increase in hexose transport. The muscle-, brain-, and liver-type hexose carriers normally expressed in tissues with different responses to insulin exhibited the same insulin sensitivity when expressed in oocytes. This was observed whether or not the insulin signal was transduced through a coexpressed human insulin receptor or the endogenous oocyte insulin-like growth factor I receptor. We conclude that the expressed human insulin receptor is able to couple efficiently with preexisting postreceptor regulatory pathways in oocytes and that the regulation of hexose transport in these cells can be mediated through the combined actions of the expressed human insulin receptor and the endogenous oocyte insulin-like growth factor I receptor.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1688999-2440870,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1688999-2479821,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1688999-2536700,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1688999-2545707,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1688999-2548842,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1688999-2644284,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1688999-2645527,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1688999-2649253,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1688999-2649883,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1688999-2649887,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1688999-2654938,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1688999-2656669,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1688999-2656686,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1688999-2656715,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1688999-2715185,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1688999-2716044,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1688999-2840662,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1688999-2842060,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1688999-2957374,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1688999-2960684,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1688999-2969892,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1688999-2971349,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1688999-3016720,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1688999-3047124,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1688999-3048704,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1688999-3100537,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1688999-3101064,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1688999-3170580,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1688999-3276714,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1688999-3285221,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1688999-3299382,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1688999-3312193,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1688999-3316205,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1688999-3399500,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1688999-3518947,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1688999-3526287,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1688999-3527041,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1688999-3839598,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1688999-3888079,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1688999-4109871,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1688999-4362069,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1688999-6266902,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1688999-6371028,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1688999-6376500,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1688999-6771756,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1688999-6865898,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1688999-7014557,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1688999-7021559,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1688999-7240211
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0270-7306
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
10
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
743-51
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:1688999-Animals,
pubmed-meshheading:1688999-Brain,
pubmed-meshheading:1688999-Gene Expression Regulation,
pubmed-meshheading:1688999-Genes,
pubmed-meshheading:1688999-Insulin,
pubmed-meshheading:1688999-Kinetics,
pubmed-meshheading:1688999-Liver,
pubmed-meshheading:1688999-Microinjections,
pubmed-meshheading:1688999-Monosaccharide Transport Proteins,
pubmed-meshheading:1688999-Muscles,
pubmed-meshheading:1688999-Oocytes,
pubmed-meshheading:1688999-Phosphorylation,
pubmed-meshheading:1688999-Protein-Tyrosine Kinases,
pubmed-meshheading:1688999-RNA,
pubmed-meshheading:1688999-Rats,
pubmed-meshheading:1688999-Receptor, Insulin,
pubmed-meshheading:1688999-Signal Transduction,
pubmed-meshheading:1688999-Transcription, Genetic,
pubmed-meshheading:1688999-Xenopus laevis
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pubmed:year |
1990
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pubmed:articleTitle |
Reconstitution of an insulin signaling pathway in Xenopus laevis oocytes: coexpression of a mammalian insulin receptor and three different mammalian hexose transporters.
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