Linked Life Data

16873765

Source:http://linkedlifedata.com/resource/pubmed/id/16873765

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2006-10-30
pubmed:abstractText
Dental pulp has the potential to form dentin as a regenerative response to caries. This regeneration is mediated by stem/progenitor cells. Thus, stem cell therapy might be of potential utility in induction of reparative dentin. We isolated side population (SP) cells from dental pulp based on the exclusion of the DNA binding dye Hoechst 33342 by flow cytometry and compared its self-renewal capacities and multipotency with non-SP cells and primary pulp cells. The cumulative cell number of the SP cells was greater than the non-SP cells and primary pulp cells. Bmi1 was continuously expressed in SP cells, suggesting longer proliferative lifespan and self-renewal capacity of SP cells. Next, the maintenance of the multilineage differentiation potential of pulp SP cells was investigated. Expression of type II collagen and aggrecan confirmed chondrogenic conversion (30%) of SP cells. SP cells expressed peroxisome proliferator-activated receptor gamma and adaptor protein 2, showing adipogenic conversion. Expression of mRNA and proteins of neurofilament and neuromodulin confirmed neurogenic conversion (90%). These results demonstrate that pulp SP cells maintain multilineage differentiation potential. We further examined whether bone morphogenetic protein 2 (BMP2) could induce differentiation of pulp SP cells into odontoblasts. BMP2 stimulated the expression of dentin sialophosphoprotein (Dspp) and enamelysin in three-dimensional pellet cultures. Autogenous transplantation of the Bmp2-supplemented SP cells on the amputated pulp stimulated the reparative dentin formation. Thus, adult pulp contains SP cells, which are enriched for stem cell properties and useful for cell therapy with BMP2 for dentin regeneration.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1066-5099
pubmed:author
pubmed:issnType
Print
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2493-503
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:16873765-Adipogenesis, pubmed-meshheading:16873765-Adolescent, pubmed-meshheading:16873765-Adult, pubmed-meshheading:16873765-Animals, pubmed-meshheading:16873765-Bone Morphogenetic Protein 2, pubmed-meshheading:16873765-Bone Morphogenetic Proteins, pubmed-meshheading:16873765-Cattle, pubmed-meshheading:16873765-Cell Differentiation, pubmed-meshheading:16873765-Cell Lineage, pubmed-meshheading:16873765-Cell Proliferation, pubmed-meshheading:16873765-Cell Separation, pubmed-meshheading:16873765-Cells, Cultured, pubmed-meshheading:16873765-Chondrogenesis, pubmed-meshheading:16873765-Dental Pulp, pubmed-meshheading:16873765-Dental Pulp Cavity, pubmed-meshheading:16873765-Dentinogenesis, pubmed-meshheading:16873765-Dogs, pubmed-meshheading:16873765-Flow Cytometry, pubmed-meshheading:16873765-Humans, pubmed-meshheading:16873765-Immunophenotyping, pubmed-meshheading:16873765-Multipotent Stem Cells, pubmed-meshheading:16873765-Neurons, pubmed-meshheading:16873765-Odontoblasts, pubmed-meshheading:16873765-Organ Culture Techniques, pubmed-meshheading:16873765-Recombinant Proteins, pubmed-meshheading:16873765-Swine, pubmed-meshheading:16873765-Transforming Growth Factor beta
pubmed:year
2006
pubmed:articleTitle
Side population cells isolated from porcine dental pulp tissue with self-renewal and multipotency for dentinogenesis, chondrogenesis, adipogenesis, and neurogenesis.
pubmed:affiliation
Laboratory of Oral Disease Research, National Institute for Longevity Sciences, National Center for Geriatrics and Gerontology, Aichi, Japan.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't